2. Academic Validation
  3. miR-96-5p is involved in alcohol-induced apoptosis in PC12 cells via negatively regulating TAp73

miR-96-5p is involved in alcohol-induced apoptosis in PC12 cells via negatively regulating TAp73

  • PLoS One. 2023 Apr 26;18(4):e0282488. doi: 10.1371/journal.pone.0282488.
Bin Yang 1 2 3,  Qi Wang 1 2,  Yanzhong Li 1 2,  Lin Li 1 2,  YanJie Zhang 1 2,  Mohammad Farris Iman Leong Bin Abdullah 3,  Wei Hao 1 2,  Duan Li 1 4,  Ruiling Zhang 1 2
Affiliations

Affiliations

  • 1 The Second Affiliated Hospital of Xinxiang Medical University, Henan Mental Hospital, Xinxiang, Henan, China.
  • 2 Henan Key Lab of Biological Psychiatry, International Joint Research Laboratory for Psychiatry and Neuroscience of Henan, Xinxiang, Henan, China.
  • 3 Department of Community Health, Advanced Medical and Dental Institute, Universiti Sains Malaysia, Kepala Batas, Pulau Pinang, Malaysia.
  • 4 Department of Microbiology, School of Basic Medical Sciences, Xinxiang Medical University, Xinxiang, Henan, China.
PMID: 37099528 DOI: 10.1371/journal.pone.0282488
Abstract

Objective: The present study opted for the adrenal phaeochromocytoma (PC12) cell line to frame a neuronal injury model induced by alcohol exposure in vitro, aiming to probe whether TAp73 and miR-96-5p are involved in the neuronal injury process induced by alcohol and elucidate the regulatory relationship between miR-96-5p and TAp73.

Methods: Immunofluorescence staining was used to observe the structural features of PC12 cells after culturing in medium with nerve growth factor (NGF). After different doses and different durations of alcohol treatment, CCK-8 assay was performed to detect the viability of PC12 cells, flow cytometry assay was carried out to detect the Apoptosis rate of PC12 cells, dual-luciferase reporter assay was used to definitude the regulatory relationship between miR-96-5p and Tp73, and western blot was used to detect the protein expression of TAp73.

Results: The result of immunofluorescence staining demonstrated that PC12 cells abundantly expressed Map2, CCK-8 assay illustrated alcohol exposure significantly downregulated the cell viability of PC12 cells, Treatment with miR-96-5p inhibitor induced Apoptosis and upregulated the expression of TAp73 in PC12 cells. Contrastingly, miR-96-5p mimic reversed the above effects and downregulation of TAp73 inhibited the Apoptosis of PC12 cells.

Conclusion: The present study demonstrated that miR-96-5p participates in alcohol-induced Apoptosis in PC12 cells via negatively regulating TAp73.

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