2. Academic Validation
  3. Maternal NAT10 orchestrates oocyte meiotic cell-cycle progression and maturation in mice

Maternal NAT10 orchestrates oocyte meiotic cell-cycle progression and maturation in mice

  • Nat Commun. 2023 Jun 22;14(1):3729. doi: 10.1038/s41467-023-39256-0.
Xue Jiang # 1,  Yu Cheng # 2,  Yuzhang Zhu # 3,  Caoling Xu # 1,  Qiaodan Li 4,  Xuemei Xing 5,  Wenqing Li 1,  Jiaqi Zou 1,  Lan Meng 1,  Muhammad Azhar 1,  Yuzhu Cao 5,  Xianhong Tong 5,  Weibing Qin 6,  Xiaoli Zhu 7,  Jianqiang Bao 8 9
Affiliations

Affiliations

  • 1 Reproductive and Genetic Hospital, the First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China (USTC), 230001, Hefei, Anhui, China.
  • 2 School of Information Science and Technology, University of Science and Technology of China (USTC), 230001, Hefei, Anhui, China.
  • 3 Division of Life Sciences and Medicine, University of Science and Technology of China (USTC), 230001, Hefei, Anhui, China.
  • 4 Laboratory animal center, University of Science and Technology of China (USTC), 230001, Hefei, Anhui, China.
  • 5 Reproductive and Genetic Hospital, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China (USTC), 230001, Hefei, Anhui, China.
  • 6 NHC Key Laboratory of Male Reproduction and Genetics, Guangdong Provincial Reproductive Science Institute (Guangdong Provincial Fertility Hospital), 510600, Guangzhou, China. guardqin@163.com.
  • 7 Reproductive and Genetic Hospital, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China (USTC), 230001, Hefei, Anhui, China. xiaolizh@ustc.edu.cn.
  • 8 Reproductive and Genetic Hospital, the First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China (USTC), 230001, Hefei, Anhui, China. jqbao@ustc.edu.cn.
  • 9 Hefei National Research Center for Physical Sciences at the Microscale, Biomedical Sciences and Health Laboratory of Anhui Province, University of Science and Technology of China (USTC), 230001, Hefei, Anhui, China. jqbao@ustc.edu.cn.
  • # Contributed equally.
PMID: 37349316 DOI: 10.1038/s41467-023-39256-0
Abstract

In mammals, the production of mature oocytes necessitates rigorous regulation of the discontinuous meiotic cell-cycle progression at both the transcriptional and post-transcriptional levels. However, the factors underlying this sophisticated but explicit process remain largely unclear. Here we characterize the function of N-acetyltransferase 10 (Nat10), a writer for N4-acetylcytidine (ac4C) on RNA molecules, in mouse oocyte development. We provide genetic evidence that Nat10 is essential for oocyte meiotic prophase I progression, oocyte growth and maturation by sculpting the maternal transcriptome through timely degradation of poly(A) tail mRNAs. This is achieved through the ac4C deposition on the key CCR4-NOT complex transcripts. Importantly, we devise a method for examining the poly(A) tail length (PAT), termed Hairpin Adaptor-poly(A) tail length (HA-PAT), which outperforms conventional methods in terms of cost, sensitivity, and efficiency. In summary, these findings provide genetic evidence that unveils the indispensable role of maternal Nat10 in oocyte development.

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