2. Academic Validation
  3. Fibrin glue delivery system containing rhein ameliorates intervertebral disc degeneration by anti-inflammatory efficacy

Fibrin glue delivery system containing rhein ameliorates intervertebral disc degeneration by anti-inflammatory efficacy

  • J Orthop Surg Res. 2023 Jul 6;18(1):485. doi: 10.1186/s13018-023-03961-9.
Jianhang Bao # 1,  Wenshuo Gao # 1,  Wei Zhang 1,  Dong Wang 2 3 4,  Hao Pan 5 6
Affiliations

Affiliations

  • 1 Department of Orthopaedics, Hangzhou TCM Hospital Affiliated to Zhejiang Chinese Medical University (Hangzhou Hospital of Traditional Chinese Medicine), No. 453 Tiyuchang Road, Xihu District, Hangzhou, 310007, Zhejiang Province, People's Republic of China.
  • 2 Department of Orthopaedics, Hangzhou TCM Hospital Affiliated to Zhejiang Chinese Medical University (Hangzhou Hospital of Traditional Chinese Medicine), No. 453 Tiyuchang Road, Xihu District, Hangzhou, 310007, Zhejiang Province, People's Republic of China. hz-wdong@alu.zcmu.edu.cn.
  • 3 Department of Orthopaedics, Hangzhou Dingqiao Hospital, No. 1630 Huanding Road, Shangcheng District, Hangzhou, 310021, Zhejiang Province, People's Republic of China. hz-wdong@alu.zcmu.edu.cn.
  • 4 Institute of Orthopaedics and Traumatology, Hangzhou TCM Hospital Affiliated to Zhejiang Chinese Medical University, No. 453 Tiyuchang Road, Xihu District, Hangzhou, 310007, People's Republic of China. hz-wdong@alu.zcmu.edu.cn.
  • 5 Department of Orthopaedics, Hangzhou TCM Hospital Affiliated to Zhejiang Chinese Medical University (Hangzhou Hospital of Traditional Chinese Medicine), No. 453 Tiyuchang Road, Xihu District, Hangzhou, 310007, Zhejiang Province, People's Republic of China. harper1966@alu.zcmu.edu.cn.
  • 6 Institute of Orthopaedics and Traumatology, Hangzhou TCM Hospital Affiliated to Zhejiang Chinese Medical University, No. 453 Tiyuchang Road, Xihu District, Hangzhou, 310007, People's Republic of China. harper1966@alu.zcmu.edu.cn.
  • # Contributed equally.
PMID: 37415165 DOI: 10.1186/s13018-023-03961-9
Abstract

Purpose: To construct an injectable, sustained-release fibrin gel containing rhein to solve the problem of low bioavailability of rhein, and observe its efficacy in the treatment of intervertebral disc degeneration.

Methods: The fibrin gel containing rhein was first synthesized in advance. Subsequently, the Materials were characterized by various experimental methods. Secondly, the degenerative cell model was constructed by stimulating nucleus pulposus cells with lipopolysaccharide (LPS), and the corresponding intervention treatment was carried out to observe the effect in vitro. Finally, the rat tail intervertebral disc was acupunctured by needles to establish the intervertebral disc degeneration model, and the effect of the material was observed through intradiscal injection.

Results: The fibrin glue containing rhein (rhein@FG) showed good injectability, sustained release and biocompatibility. Rhein@FG can improve the LPS-induced inflammatory microenvironment, regulate ECM metabolic disorders of nucleus pulposus cells and aggregation of the NLRP3 inflammasome in vitro, and inhibit cell Pyroptosis. Furthermore, in vivo experiments, rhein@FG effectively prevented needle puncture-induced intervertebral disc degeneration in rats.

Conclusions: Rhein@FG has better efficacy than rhein or FG alone due to its slow release and mechanical properties, which can be used as a potential replacement therapy for intervertebral disc degeneration.

Keywords

Drug delivery system; Fibrin gel; Intervertebral disc degeneration; Pyroptosis; Rhein.

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