2. Academic Validation
  3. Escherichia coli Nissle 1917 ghosts alleviate inflammatory bowel disease in zebrafish

Escherichia coli Nissle 1917 ghosts alleviate inflammatory bowel disease in zebrafish

  • Life Sci. 2023 Jul 19;329:121956. doi: 10.1016/j.lfs.2023.121956.
Haojie Chen 1,  Pengyu Lei 1,  Hao Ji 1,  Jiahui Ma 1,  Yimeng Fang 1,  Haiyang Yu 1,  Jiao Du 1,  Linkai Qu 2,  Qinsi Yang 3,  Li Luo 4,  Kun Zhang 5,  Wei Wu 6,  Libo Jin 7,  Da Sun 8
Affiliations

Affiliations

  • 1 Institute of Life Sciences & Biomedical Collaborative Innovation Center of Zhejiang Province, Wenzhou University, Wenzhou 325035, China.
  • 2 College of Veterinary Medicine, Jilin University, Changchun 130062, China.
  • 3 Wenzhou Institute, University of Chinese Academy of Sciences, Wenzhou 325000, China.
  • 4 Affiliated Dongguan Hospital, Southern Medical University, Dongguan 523059, China.
  • 5 Key Laboratory for Biorheological Science and Technology of Ministry of Education, State and Local Joint Engineering Laboratory for Vascular Implants, Bioengineering College of Chongqing University, Chongqing 400044, China.
  • 6 Key Laboratory for Biorheological Science and Technology of Ministry of Education, State and Local Joint Engineering Laboratory for Vascular Implants, Bioengineering College of Chongqing University, Chongqing 400044, China. Electronic address: david2015@cqu.edu.cn.
  • 7 Institute of Life Sciences & Biomedical Collaborative Innovation Center of Zhejiang Province, Wenzhou University, Wenzhou 325035, China. Electronic address: 20160121@wzu.edu.cn.
  • 8 Institute of Life Sciences & Biomedical Collaborative Innovation Center of Zhejiang Province, Wenzhou University, Wenzhou 325035, China. Electronic address: sunday@wzu.edu.cn.
PMID: 37473802 DOI: 10.1016/j.lfs.2023.121956
Abstract

Escherichia coli Nissle 1917 (EcN) has become a research hotspot in inflammatory bowel disease (IBD). It has a strong targeting effect on the colon, and has some therapeutic effect on inflammatory bowel disease. EcN is prepared into EcN ghosts, which also retain EcN's biological characteristics. Consequently, EcN ghosts are used for Drug Delivery. This study evaluated the safety and efficacy of EcN ghosts as carriers of drugs for treating IBD in zebrafish. Caco-2 cell adhesion experiments and zebrafish intestinal adhesion experiments demonstrated that EcN ghosts was highly adherent to the intestine. Additionally, oral administration of EcN ghosts attenuated dextran sulfate sodium-induced IBD symptoms by inhibiting neutrophil chemotaxis and Reactive Oxygen Species production in larval zebrafish. Because of the unique biological functions of EcN ghosts, it may serve as a strategy for future targeted Drug Delivery in IBD treatment.

Keywords

Bacterial ghosts; Drug delivery system; Escherichia coli Nissle 1917; Inflammatory bowel disease; Zebrafish.

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