2. Academic Validation
  3. Anti-metastatic effect of taraxasterol on prostate cancer cell lines

Anti-metastatic effect of taraxasterol on prostate cancer cell lines

  • Res Pharm Sci. 2023 Jun 1;18(4):439-448. doi: 10.4103/1735-5362.378090.
Morteza Movahhed 1,  Mona Pazhouhi 2,  Hadi Esmaeili Gouvarchin Ghaleh 3,  Bahman Jalali Kondori 4 5
Affiliations

Affiliations

  • 1 Department of Pathology, Baqiyatallah University of Medical Sciences, Tehran, Iran.
  • 2 Medical Biology Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, I.R. Iran.
  • 3 Applied Virology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
  • 4 Department of Anatomical Sciences, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran.
  • 5 Baqiyatallah Research Center for Gastroenterology and Liver Diseases (BRCGL), Baqiyatallah University of Medical Sciences, Tehran, Iran.
PMID: 37614618 DOI: 10.4103/1735-5362.378090
Abstract

Background and purpose: Prostate Cancer is the second cause of death among men. Nowadays, treating various cancers with medicinal Plants is more common than Other therapeutic agents due to their minor side effects. This study aimed to evaluate the effect of taraxasterol on the prostate Cancer cell line.

Experimental approach: The prostate Cancer cell line (PC3) was cultured in a nutrient medium. MTT method and trypan blue staining were used to evaluate the viability of cells in the presence of different concentrations of taraxasterol, and IC50 was calculated. Real-Time PCR was used to measure the expression of MMP-9, MMP-2, uPA, uPAR, TIMP-2, and TIMP-1 genes. Gelatin zymography was used to determine MMP-9 and MMP-2 Enzyme activity levels. Finally, the effect of taraxasterol on cell invasion, migration, and adhesion was investigated.

Findings/results: Taraxasterol decreased the survival rate of PC3 cells at IC50 time-dependently (24, 48, and 72 h). Taraxasterol reduced the percentage of PC3 cell adhesion, invasion, and migration by 74, 56, and 76 percent, respectively. Real-Time PCR results revealed that uPA, uPAR, MMP-9, and MMP-2 gene expressions decreased in the taraxasterol-treated groups, but TIMP-2 and TIMP-1 gene expressions increased significantly. Also, a significant decrease in the level of MMP-9 and MMP-2 Enzymes was observed in the PC3 cell line treated with taraxasterol.

Conclusion and implications: The present study confirmed the therapeutic role of taraxasterol in preventing prostate Cancer cell metastasis in the in-vitro study.

Keywords

MMP-2; MMP-9; PC3 cell; Prostate cancer; Taraxasterol.

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