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  3. A flow-cytometry-based pipeline for the rapid quantification of C2C12 cell differentiation

A flow-cytometry-based pipeline for the rapid quantification of C2C12 cell differentiation

  • STAR Protoc. 2023 Oct 10;4(4):102637. doi: 10.1016/j.xpro.2023.102637.
Bianca Parisi 1,  Maxime Sünnen 1,  Rohan Chippalkatti 2,  Daniel Kwaku Abankwa 3
Affiliations

Affiliations

  • 1 Cancer Cell Biology and Drug Discovery Group, Department of Life Sciences and Medicine, University of Luxembourg, 4362 Esch-sur-Alzette, Luxembourg.
  • 2 Cancer Cell Biology and Drug Discovery Group, Department of Life Sciences and Medicine, University of Luxembourg, 4362 Esch-sur-Alzette, Luxembourg. Electronic address: rohan.chippalkatti@uni.lu.
  • 3 Cancer Cell Biology and Drug Discovery Group, Department of Life Sciences and Medicine, University of Luxembourg, 4362 Esch-sur-Alzette, Luxembourg. Electronic address: daniel.abankwa@uni.lu.
PMID: 37819762 DOI: 10.1016/j.xpro.2023.102637
Abstract

The C2C12 cell line represents a simple in vitro model for cell differentiation. Here, we present a flow-cytometry-based pipeline to quantitate C2C12 cell differentiation based on Myosin heavy-chain marker expression. We describe steps for cell seeding, transfection, drug treatment, differentiation, and labeling. We then detail procedures for flow cytometry acquisition and introduce the R script FlowFate for automated analysis, including the study of dose-dependent effects of GFP-tagged genes on differentiation. For complete details on the use and execution of this protocol, please refer to Chippalkatti et al. (2023).1.

Keywords

Cancer; Cell Biology; Molecular Biology.

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