2. Academic Validation
  3. Corydalis Saxicola Bunting Total Alkaloid Eliminates Porphyromonas gingivalis strain 33277 Internalized into Macrophages by Inhibition of TLR2

Corydalis Saxicola Bunting Total Alkaloid Eliminates Porphyromonas gingivalis strain 33277 Internalized into Macrophages by Inhibition of TLR2

  • Microbes Infect. 2023 Oct 30:105244. doi: 10.1016/j.micinf.2023.105244.
Lan Yang 1,  Jiaxuan Wu 2,  Guocheng Mei 3,  Qiaozhi Jiang 4,  Zhiheng Su 5,  Haiqing Liao 6,  Zhenmin Liu 7,  Renchuan Tao 8,  Xiangzhi Yong 9
Affiliations

Affiliations

  • 1 Department of Periodontics and Oral Medicine, College of Stomatology, Guangxi Medical University, Nanning, China; Guangxi Health Commission Key Laboratory of Prevention and Treatment for Oral Infectious Diseases, Nanning, China; Guangxi Key Laboratory of Oral and Maxillofacial Rehabilitation and Reconstruction, Guangxi Universities and Colleges Key Laboratory of Oral and Maxillofacial Surgery Disease Treatment, Guangxi Clinical Research Center for Craniofacial Deformity, Nanning, China. Electronic address: 1215395049@qq.com.
  • 2 Department of Periodontics and Oral Medicine, College of Stomatology, Guangxi Medical University, Nanning, China; Guangxi Health Commission Key Laboratory of Prevention and Treatment for Oral Infectious Diseases, Nanning, China; Guangxi Key Laboratory of Oral and Maxillofacial Rehabilitation and Reconstruction, Guangxi Universities and Colleges Key Laboratory of Oral and Maxillofacial Surgery Disease Treatment, Guangxi Clinical Research Center for Craniofacial Deformity, Nanning, China. Electronic address: wujiaxuan7@163.com.
  • 3 Department of Periodontics and Oral Medicine, College of Stomatology, Guangxi Medical University, Nanning, China; Guangxi Health Commission Key Laboratory of Prevention and Treatment for Oral Infectious Diseases, Nanning, China; Guangxi Key Laboratory of Oral and Maxillofacial Rehabilitation and Reconstruction, Guangxi Universities and Colleges Key Laboratory of Oral and Maxillofacial Surgery Disease Treatment, Guangxi Clinical Research Center for Craniofacial Deformity, Nanning, China. Electronic address: 304234138@qq.com.
  • 4 Department of Periodontics and Oral Medicine, College of Stomatology, Guangxi Medical University, Nanning, China; Guangxi Health Commission Key Laboratory of Prevention and Treatment for Oral Infectious Diseases, Nanning, China; Guangxi Key Laboratory of Oral and Maxillofacial Rehabilitation and Reconstruction, Guangxi Universities and Colleges Key Laboratory of Oral and Maxillofacial Surgery Disease Treatment, Guangxi Clinical Research Center for Craniofacial Deformity, Nanning, China.
  • 5 Pharmaceutical College, Guangxi Medical University, Nanning, China. Electronic address: suzhiheng1981@126.com.
  • 6 Department of Periodontics and Oral Medicine, College of Stomatology, Guangxi Medical University, Nanning, China; Guangxi Health Commission Key Laboratory of Prevention and Treatment for Oral Infectious Diseases, Nanning, China; Guangxi Key Laboratory of Oral and Maxillofacial Rehabilitation and Reconstruction, Guangxi Universities and Colleges Key Laboratory of Oral and Maxillofacial Surgery Disease Treatment, Guangxi Clinical Research Center for Craniofacial Deformity, Nanning, China. Electronic address: liaohaiqing12@126.com.
  • 7 Department of Periodontics and Oral Medicine, College of Stomatology, Guangxi Medical University, Nanning, China; Guangxi Health Commission Key Laboratory of Prevention and Treatment for Oral Infectious Diseases, Nanning, China; Guangxi Key Laboratory of Oral and Maxillofacial Rehabilitation and Reconstruction, Guangxi Universities and Colleges Key Laboratory of Oral and Maxillofacial Surgery Disease Treatment, Guangxi Clinical Research Center for Craniofacial Deformity, Nanning, China. Electronic address: 1149054376@qq.com.
  • 8 Department of Periodontics and Oral Medicine, College of Stomatology, Guangxi Medical University, Nanning, China; Guangxi Health Commission Key Laboratory of Prevention and Treatment for Oral Infectious Diseases, Nanning, China; Guangxi Key Laboratory of Oral and Maxillofacial Rehabilitation and Reconstruction, Guangxi Universities and Colleges Key Laboratory of Oral and Maxillofacial Surgery Disease Treatment, Guangxi Clinical Research Center for Craniofacial Deformity, Nanning, China. Electronic address: taorecnhuan@gxmu.edu.cn.
  • 9 Department of Periodontics and Oral Medicine, College of Stomatology, Guangxi Medical University, Nanning, China; Guangxi Health Commission Key Laboratory of Prevention and Treatment for Oral Infectious Diseases, Nanning, China; Guangxi Key Laboratory of Oral and Maxillofacial Rehabilitation and Reconstruction, Guangxi Universities and Colleges Key Laboratory of Oral and Maxillofacial Surgery Disease Treatment, Guangxi Clinical Research Center for Craniofacial Deformity, Nanning, China. Electronic address: yongyongsyl@qq.com.
PMID: 37914020 DOI: 10.1016/j.micinf.2023.105244
Abstract

Objective: This study aimed to investigate the impact of Corydalis Saxicola Bunting Total Alkaloid (CSBTA) on Porphyromonas gingivalis internalization within macrophages and explore the potential role of Toll-like Receptor 2 (TLR2) in this process.

Methods: We established a P. gingivalis internalization model in macrophages by treating P. gingivalis-infected macrophages (MOI=100:1) with 200 μg/mL metronidazole and 300 μg/mL gentamicin for 1 hour. Subsequently, the model was exposed to CSBTA at concentrations of 0.02 g/L or 1 μg/mL Pam3CSK4. After a 6-hour treatment, Cell Lysis was performed with sterile water to quantify Bacterial colonies. The mRNA expressions of TLR2 and IL-8 in macrophages were analyzed using RT-qPCR, while their protein levels were assessed via Western blot and ELISA respectively.

Results: P. gingivalis could internalize into macrophages and enhance the expression of TLR2 and IL-8. Activation of TLR2 by Pam3CSK4 contributed to P. gingivalis survival within macrophages and increased TLR2 and IL-8 expression. Conversely, 0.02 g/L CSBTA effectively cleared intracellular P. gingivalis, achieving a 90% clearance rate after 6 hours. Moreover, it downregulated the expression of TLR2 and IL-8 induced by P. gingivalis. However, the inhibitory effect of CSBTA on the internalized P. gingivalis model was attenuated by Pam3CSK4.

Conclusion: CSBTA exhibited the ability to reduce the presence of live intracellular P. gingivalis and lower IL-8 expression in macrophages, possibly by modulating TLR2 activity.

Keywords

Corydalis Saxicola Bunting Total Alkaloid; Porphyromonas gingivalis; interleukin-8; macrophages; toll-like receptor 2.

Figures
Products