2. Academic Validation
  3. Structural identification of an polysaccharide isolated from Epimedium brevicornum and its beneficial effect on promoting osteogenesis in osteoblasts induced by high glucose

Structural identification of an polysaccharide isolated from Epimedium brevicornum and its beneficial effect on promoting osteogenesis in osteoblasts induced by high glucose

  • Biomed Pharmacother. 2023 Nov 16:169:115893. doi: 10.1016/j.biopha.2023.115893.
Shan Shan Lei 1,  Bo Li 2,  Xiao Wen Huang 1,  Xu Pin Wang 1,  Shan Xiong 3,  Rui Duan 4,  Lin Zi Li 5
Affiliations

Affiliations

  • 1 Department of Medicine, Zhejiang Academy of Traditional Chinese Medicine, Hangzhou, Zhejiang 310007, PR China.
  • 2 Zhejiang University of Technology, Hangzhou, Zhejiang 310014, PR China.
  • 3 School of Pharmaceutical Sciences & Institute of Materia Medica, Shandong First Medical University & Shandong Academy of Medical Sciences, National Key Laboratory of Advanced Drug Delivery System, Key Laboratory for Biotechnology Drugs of National Health Commission (Shandong Academy of Medical Sciences), Key Lab for Rare & Uncommon Diseases of Shandong Province, Jinan, Shandong 250117, PR China. Electronic address: shanxiong83@sohu.com.
  • 4 Jingmen Central Hospital, Jingmen, Hubei 448000, PR China. Electronic address: duanrui@sina.com.
  • 5 Jingmen Central Hospital, Jingmen, Hubei 448000, PR China. Electronic address: lilinzi1989@163.com.
PMID: 37979377 DOI: 10.1016/j.biopha.2023.115893
Abstract

Aim: Diabetes osteoporosis (DOP) is a chronic bone Metabolic Disease induced by diabetes, whose morbidity continues to increase. Epimedium brevicornum Maxim (EB), a popular Chinese traditional medicine, has been used to treat bone diseases in China for thousands of years. But its material basis and specific mechanism of action are not clear.

Methods: Epimedium brevicornum crude polysaccharide (EPE) is the main component, in this research the characterized the structure of EBPC1 purified from EPE was detected and its effects on cell proliferation, differentiation, and cytoskeletal in osteoblasts induced by high glucose.

Results: The molecular weight of EBPC1 was 10.5 kDa. It was mainly comprised of glucose and galactose, and the backbone of EBPC1 was→4)-α-D-Galp-(1→4)-α-D-Galp-(1→6)-β-D-Galp-(1→6)-β-D-Galp-(1→4)-α-D-Glcp-(1→4)-α-D-Glcp-(1→. The results from in vitro experiments revealed that EBPC1 significantly increased Alkaline Phosphatase (ALP) activity and mineralized nodule formation in primary osteoblasts, also significantly up-regulated expression of Alp mRNA and Runx2 mRNA in the presence of EBPC1 pretreatment. Moreover, EBPC1 modulated Apoptosis via the regulation of Bax/Bcl2.

Conclusion: These results indicate that EBPC1 treatment can promote osteogenesis during DOP, which can ameliorate Apoptosis by regulating Bax/Bcl2 and accelerating osteogenesis in osteoblasts.

Keywords

Diabetes osteoporosis; Epimedium brevicornum polysaccharide; Pharmacological mechanisms; Structural resolution.

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