2. Academic Validation
  3. CLIC3 interacts with NAT10 to inhibit N4-acetylcytidine modification of p21 mRNA and promote bladder cancer progression

CLIC3 interacts with NAT10 to inhibit N4-acetylcytidine modification of p21 mRNA and promote bladder cancer progression

  • Cell Death Dis. 2024 Jan 5;15(1):9. doi: 10.1038/s41419-023-06373-z.
Yujun Shuai # 1,  Hui Zhang # 1,  Changhao Liu # 2,  Junting Wang 2,  Yangkai Jiang 1,  Jiayin Sun 1,  Xincheng Gao 1,  Xiaochen Bo 2,  Xingyuan Xiao 3,  Xin Liao 4,  Chao Huang 5,  Hebing Chen 6,  Guosong Jiang 7
Affiliations

Affiliations

  • 1 Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.
  • 2 Institute of Health Service and Transfusion Medicine, Beijing, 100850, China.
  • 3 Department of Urology, Zhongnan Hospital of Wuhan University, Wuhan, 430071, China.
  • 4 Department of General Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
  • 5 Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China. huangchao1009@163.com.
  • 6 Institute of Health Service and Transfusion Medicine, Beijing, 100850, China. chenhb@bmi.ac.cn.
  • 7 Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China. jiangguosongdoc@hotmail.com.
  • # Contributed equally.
PMID: 38182571 DOI: 10.1038/s41419-023-06373-z
Abstract

Chromatin accessibility plays important roles in revealing the regulatory networks of gene expression, while its application in bladder Cancer is yet to be fully elucidated. Chloride intracellular channel 3 (CLIC3) protein has been reported to be associated with the progression of some tumors, whereas the specific mechanism of CLIC3 in tumor remains unclear. Here, we screened for key genes in bladder Cancer through the identification of transcription factor binding site clustered region (TFCR) on the basis of chromatin accessibility and TF motif. CLIC3 was identified by joint profiling of chromatin accessibility data with TCGA database. Clinically, CLIC3 expression was significantly elevated in bladder Cancer and was negatively correlated with patient survival. CLIC3 promoted the proliferation of bladder Cancer cells by reducing p21 expression in vitro and in vivo. Mechanistically, CLIC3 interacted with NAT10 and inhibited the function of NAT10, resulting in the downregulation of ac4C modification and stability of p21 mRNA. Overall, these findings uncover an novel mechanism of mRNA ac4C modification and CLIC3 may act as a potential therapeutic target for bladder Cancer.

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