2. Academic Validation
  3. Puerarin alleviates diabetic nephropathy by inhibiting Caspase-1-mediated pyroptosis

Puerarin alleviates diabetic nephropathy by inhibiting Caspase-1-mediated pyroptosis

  • J Pharm Pharmacol. 2024 Jan 12:rgad113. doi: 10.1093/jpp/rgad113.
QiuYan Chen 1 2,  Lu Wang 1 2,  Xiaojie Wei 3,  Ming Chen 4 5,  Xiaoping Zhang 1 2,  Rou Mo 1 2,  Renbin Huang 1 2,  Tao Liang 4 5,  Xiaohui Xu 1 2
Affiliations

Affiliations

  • 1 Pharmaceutical College, Guangxi Medical University, No. 22, Shuangyong Road, Nanning, Guangxi 530021, PR China.
  • 2 Department of Pharmacy, Guangxi Medical University Cancer Hospital, No. 71, Hedi Road, Nanning, Guangxi 530021, PR China.
  • 3 College of Basic Medicine, Guangxi University of Chinese Medicine, No. 13, Wuhe Road, Nanning, Guangxi 530200, PR China.
  • 4 College & Hospital of Stomatology, Guangxi Medical University, No. 10, Shuangyong Road, Nanning, Guangxi 530021, PR China.
  • 5 Key Laboratory of Research and Application of Stomatological Equipment (College of Stomatology, Hospital of Stomatology, Guangxi Medical University), Education Department of Guangxi Zhuang Autonomous Region, No. 10, Shuangyong Road, Nanning, Guangxi 530021, PR China.
PMID: 38215026 DOI: 10.1093/jpp/rgad113
Abstract

Background and purpose: Diabetic nephropathy (DN) is an important cause of end-stage renal disease, with podocyte injury as the main feature. Pyroptosis plays a non-negligible role in the process of diabetic nephropathy. Puerarin (PR) treatment of diabetic nephropathy has great potential, but the mechanism is not very clear. This article aims to study the protective effect and mechanism of puerarin on DN.

Methods: Streptozotocin (STZ)-induced C57 BL/6J mouse model of DN was given PR, Necrosulfomide (NSA), Nigericin for 12 weeks; A 60 mM high glucose(HG) induced MPC5 cell injury model was administered to PR, NSA, and Nigericin interventions for 24 h.

Results: After 12 weeks of administration, PR reduced fasting blood glucose levels in DN mice, alleviated glomerular lesions, reduced podocyte damage, and protected renal function. Meanwhile, PR also inhibits the expression of pyroptosis-related proteins. In addition, PR alleviated the release of Interleukin 18 (IL-18), Interleukin 1beta (IL-1β), and Lactate Dehydrogenase (LDH) in MPC5 cells under HG conditions, downregulated the expression of pyrozozois-related proteins, and improved Caspase-1-mediated Pyroptosis in MPC5 cells.

Conclusion: Our study suggests that the beneficial effects of PR in diabetic nephropathy may be associated with inhibition of Caspase-1-mediated Pyroptosis.

Keywords

MPC5; diabetic nephropathy; puerarin; pyroptosis.

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