2. Membrane Transporter/Ion Channel Immunology/Inflammation Anti-infection Apoptosis Stem Cell/Wnt
  3. Sodium Channel NOD-like Receptor (NLR) Bacterial Apoptosis Antibiotic Pyroptosis Wnt β-catenin
  4. Nigericin

Nigericin is an antibiotic derived from Streptomyces hygroscopicus that act as a K+/H+ ionophore, promoting K+/H+ exchange across mitochondrial membranes. Nigericin shows promising anti-cancer activities through decreasing intracellular pH (pHi), and inactivation of Wnt/β-catenin signals. Nigericin induces pyroptosis through caspase 1/GSDMD in TNBC.

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Nigericin Chemical Structure

Nigericin Chemical Structure

CAS No. : 28380-24-7

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Based on 111 publication(s) in Google Scholar

Other Forms of Nigericin:

Nigericin Related Antibodies

Top Publications Citing Use of Products

108 Publications Citing Use of MCE Nigericin

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    Nigericin purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2023 Mar 5;113:154743.  [Abstract]

    Priming with LPS (1 μg/mL; 4 h) and activating with Nigericin (10 μM; 2 h) induces NLRP3 expression, caspase-1 cleavage, and GSDMD cleavage in BMDMs.

    Nigericin purchased from MedChemExpress. Usage Cited in: Environ Toxicol. 2023 Mar 29.  [Abstract]

    Nigericin (1.38mM; 2 μg/2 μL; 10 min) signifcantly increases the expression of IL-1β, IL-18, and GSDMD N-terminal in rats

    Nigericin purchased from MedChemExpress. Usage Cited in: J Cell Mol Med. 2020 Jul;24(14):8078-8090.  [Abstract]

    LPS + Nigericin is used to induce pyroptosis as a positive control group.

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    Description

    Nigericin is an antibiotic derived from Streptomyces hygroscopicus that act as a K+/H+ ionophore, promoting K+/H+ exchange across mitochondrial membranes. Nigericin shows promising anti-cancer activities through decreasing intracellular pH (pHi), and inactivation of Wnt/β-catenin signals. Nigericin induces pyroptosis through caspase 1/GSDMD in TNBC[1][2][3][4][5][6][7].

    IC50 & Target

    NLRP3

     

    In Vitro

    Nigericin (0-4 μg/ml; 24 h) induces pyroptosis through caspase 1/GSDMD pathway in TNBC cells[6].
    Nigericin (1 μg/mL, 8 × MIC; 2 μg/mL, 16 × MIC; 196 h) is a bactericidal antibiotic against MDR gram-positive bacteria[4].
    Nigericin (0-0.25 μg/ml; 24 h) inactivates Wnt/β-catenin pathway in H460 cells as an anti-cancer effect[7].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Nigericin Related Antibodies

    Cell Viability Assay[4]

    Cell Line: S. aureus, Staphylococcus epidermidis, Enterococcus faecalis, E. faecium, S. pneumoniae) , and Streptococcus agalactiae
    Concentration: 0 μg/ml, 0.05 μg/ml, 0.125 μg/ml, 0.25 μg/ml
    Incubation Time: 24 h
    Result: Exhibited potent activity against these clinical MDR strains, with MIC values ranging from 0.004-0.25 mg/ml.

    Western Blot Analysis[6]

    Cell Line: MDA-MB-231, and 4T1 cells
    Concentration: 0 μg/ml, 2 μg/ml, 4 μg/ml
    Incubation Time: 24 h
    Result: Showed increasing in the proteins level of caspase 1 and N-GSDMD.

    Cell Viability Assay[7]

    Cell Line: H460 cells
    Concentration: 0.5 μM, 1 μM, 2.5 μM
    Incubation Time: 24 h
    Result: Showed downregulating in the expression of proteins of the canonical Wnt (LRP6, Wnt5a/b, and β-catenin) signaling pathway.
    In Vivo

    Nigericin (1 mg/kg; i.p.; every 12 h for 3 D) reduces the infection of S. aureus USA300 in mice[4].
    Nigericin (0.025 mg/kg; s.c.; every two days in 4 weeks) plus anti-PD-1 shows synergistic anti-cancer effect[6].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: BALB/c Mice injected orthotopically with 4T1 cells[6]
    Dosage: 0.025 mg/kg
    Administration: Subcutaneous injection (s.c.)
    Result: Showed combination with anti-PD-1 antibody almost completely suppressed tumor growth.
    Animal Model: Mice Infected with S. aureus USA300[4]
    Dosage: 1 mg/kg
    Administration: Intraperitoneal injection (i.p.)
    Result: Showed reduction in the bacterial burden to 1,000-10,000-fold in the major organs.
    Molecular Weight

    724.96

    Formula

    C40H68O11
    CAS No.
    SMILES

    C[C@H]1[C@](O[C@@H]2C[C@](CC[C@@H]3C)([H])O[C@@]3([H])[C@@H](C)C(O)=O)([C@@H]([C@H](OC)C2)C)O[C@](C)([C@]4([H])O[C@](C)([C@]5([H])O[C@]([C@@]([C@H](C[C@H]6C)C)([H])O[C@@]6(O)CO)([H])C[C@@H]5C)CC4)C1
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    Please store the product under the recommended conditions in the Certificate of Analysis.
    Purity & Documentation
    References
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    Nigericin Related Classifications

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    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Nigericin28380-24-7Sodium ChannelNOD-like Receptor (NLR)BacterialApoptosisAntibioticPyroptosisWntβ-cateninNa channelsNa+ channelsBeta cateninCancerInhibitorinhibitorinhibit

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