2. Academic Validation
  3. IGF2BP3-stabilized CAMK1 regulates the mitochondrial dynamics of renal tubule to alleviate diabetic nephropathy

IGF2BP3-stabilized CAMK1 regulates the mitochondrial dynamics of renal tubule to alleviate diabetic nephropathy

  • Biochim Biophys Acta Mol Basis Dis. 2024 Jan 10:167022. doi: 10.1016/j.bbadis.2024.167022.
Du Yuan 1,  Hao Li 1,  Wenni Dai 1,  Xun Zhou 1,  Wen Zhou 2,  Liyu He 3
Affiliations

Affiliations

  • 1 Department of Nephrology, The Second Xiangya Hospital of Central South University, Hunan Key Laboratory of Kidney Disease and Blood Purification, No. 139 People's Middle Road, Changsha 410011, Hunan, People's Republic of China.
  • 2 Clinical Nursing Teaching and Research Section, The Second Xiangya Hospital of Central South University, Changsha, People's Republic of China.
  • 3 Department of Nephrology, The Second Xiangya Hospital of Central South University, Hunan Key Laboratory of Kidney Disease and Blood Purification, No. 139 People's Middle Road, Changsha 410011, Hunan, People's Republic of China. Electronic address: heliyu1124@csu.edu.cn.
PMID: 38216068 DOI: 10.1016/j.bbadis.2024.167022
Abstract

Background: CAMK1 has been shown to be involved in human disease progression via regulating mitochondrial dynamics. However, whether CAMK1 mediates mitochondrial dynamics to regulate diabetic nephropathy (DN) process remains unclear.

Methods: Mice were injected with streptozotocin (STZ) to mimic diabetic mice models in vivo, and mice with proximal tubule-specific knockout of CAMK1 (CAMK1-KO) were generated. HK-2 cells were treated with high-glucose (HG) to mimic DN cell model in vitro. Histopathological analysis was performed to confirm kidney injury in mice. ROS production and Apoptosis were assessed by DHE staining and TUNEL staining. Mitochondria morphology was observed and analyzed by electron microscopy. Mitochondrial membrane potential was detected by JC-1 staining, and cell proliferation was measured by EdU assay. The mRNA and protein expression were examined by qRT-PCR, western blot and immunostaining. RNA interaction was confirmed by RIP assay and dual-luciferase reporter assay. The mRNA stability was tested by actinomycin D treatment, and m6A level was examined by MeRIP assay.

Results: CAMK1 was reduced in DN patients and STZ-induced diabetic mice. Conditional deletion of CAMK1 aggravated kidney injury and promoted mitochondrial fission in diabetic mice. CAMK1 overexpression inhibited mitochondrial fission to alleviate HG-induced HK-2 cell Apoptosis. IGF2BP3 promoted the stability of CAMK1 mRNA by m6A modification. IGF2BP3 inhibited mitochondrial fission to repress cell Apoptosis in vitro and kidney injury in vivo by increasing CAMK1 expression.

Conclusion: IGF2BP3-mediated CAMK1 mRNA stability alleviated DN progression by inhibiting mitochondria fission.

Keywords

CAMK1; Diabetic nephropathy; IGF2BP3; Mitochondria fission; m6A.

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