Inducible IL-2 production and IL-2+ cell expansion are landmark events for T-cell activation of teleost

As a multipotent cytokine, interleukin (IL)-2 plays important roles in activation, differentiation and survival of the lymphocytes. Although biological characteristics and function of IL-2 have been clarified in several teleost species, evidence regarding IL-2 production at the cellular and protein levels is still scarce in fish due to the lack of reliable antibody. In this study, we developed a mouse anti-Nile tilapia IL-2 monoclonal antibody (mAb), which could specifically recognize IL-2 protein and identify IL-2-producing lymphocytes of tilapia. Using this mAb, we found that CD3⁺ T cells, but not CD3^- lymphocytes, are the main cellular source of IL-2 in tilapia. Under resting condition, both CD3⁺CD4-1⁺ T cells and CD3⁺CD4-1^- T cells of tilapia produce IL-2. Moreover, the IL-2 protein level and the frequency of IL-2⁺ T cells significantly increased once T cells were activated by phytohemagglutinin (PHA) or CD3 plus CD28 mAbs in vitro. In addition, Edwardsiella piscicida Infection also induces the IL-2 production and the expansion of IL-2⁺ T cells in the spleen lymphocytes. These findings demonstrate that IL-2 takes part in the T-cell activation and anti-bacterial adaptive immune response of tilapia, and can serve as an important marker for T-cell activation of teleost fish. Our study has enriched the knowledge regarding T-cell response in fish species, and also provide novel perspective for understanding the evolution of adaptive immune system.

Adaptive immunity; Evolution; IL-2; Oreochromis niloticus; T-cell activation.