2. Academic Validation
  3. Matrine attenuating cardiomyocyte apoptosis in doxorubicin-induced cardiotoxicity through improved mitochondrial membrane potential and activation of mitochondrial respiratory chain Complex I pathway

Matrine attenuating cardiomyocyte apoptosis in doxorubicin-induced cardiotoxicity through improved mitochondrial membrane potential and activation of mitochondrial respiratory chain Complex I pathway

  • Biomed Pharmacother. 2024 Apr:173:116464. doi: 10.1016/j.biopha.2024.116464.
Chu Erfu 1,  Liu Li 2,  Qu Weiting 3,  Chi Tao 4,  Ma Liwei 5,  Yang Hemin 4,  Lu Junkun 6
Affiliations

Affiliations

  • 1 Department of Cardiology, Third Affiliated Hospital of Qiqihar Medical College, Qiqihar Medical College, Heilongjiang, China. Electronic address: ralilve@163.com.
  • 2 Department of Cardiology, First Traditional Medicine Hospital of Zhanjiang, Guangzhou University of Chinese Medicine, Guangdong, 524043, China.
  • 3 Department of Anesthesiology, Qiqihar Jianhua Hospital, Heilongjiang, China.
  • 4 Department of Central Lab, Third Affiliated Hospital of Qiqihar Medical College, Qiqihar Medical College, Heilongjiang, China.
  • 5 Department of Institute of Medine & Pharmacy, Qiqihaer Medical College, Heilongjiang, China.
  • 6 Department of Cardiology, First Traditional Medicine Hospital of Zhanjiang, Guangzhou University of Chinese Medicine, Guangdong, 524043, China. Electronic address: ljkuuu@163.com.
PMID: 38503242 DOI: 10.1016/j.biopha.2024.116464
Abstract

The study aimed to demonstrate that matrine can reduce Apoptosis in H9c2 cells induced by the cardiotoxic Anticancer drug doxorubicin (DOX).The researchers pretreated H9c2 cells with different concentrations of matrine before exposing them to DOX and cultured them for 24 h. They assessed cell survival rates using cell counting kit-8 and MTT assay. Hoechst 33258 dye kits were used to determine Apoptosis, while laser confocal JC-1 method was applied to test the mitochondrial membrane potential (MMP). Complex I activities were detected following the manufacturer's protocol. The results indicated that matrine pretreatment significantly increased the survival rate of H9c2 cells injured by DOX. Additionally, matrine reduced Apoptosis in H9c2 cells through the improvement of MMP and activity of Complex I, which were damaged by DOX.

Keywords

Apoptosis; Complex I; Doxorubicin; MMP; Matrine.

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