2. Academic Validation
  3. Non-canonical role for the BAF complex subunit DPF3 in mitosis and ciliogenesis

Non-canonical role for the BAF complex subunit DPF3 in mitosis and ciliogenesis

  • J Cell Sci. 2024 May 1;137(9):jcs261744. doi: 10.1242/jcs.261744.
Giulia Verrillo 1,  Anna Maria Obeid 1,  Alexia Genco 1,  Jacopo Scrofani 2,  François Orange 3,  Sarah Hanache 1,  Julien Mignon 4,  Tanguy Leyder 4,  Catherine Michaux 4,  Céline Kempeneers 5 6,  Noëmie Bricmont 5 6,  Stephanie Herkenne 7,  Isabelle Vernos 2 8 9,  Maud Martin 10,  Denis Mottet 1
Affiliations

Affiliations

  • 1 University of Liege, GIGA - Research Institute, Molecular Analysis of Gene Expression (MAGE) Laboratory, B34, Avenue de l'Hôpital, B-4000 Liège, Belgium.
  • 2 Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, Barcelona 08003, Spain.
  • 3 Université Côte d'Azur, Centre Commun de Microscopie Appliquée (CCMA), 06100 Nice, France.
  • 4 University of Namur, Laboratory of Physical Chemistry of Biomolecules, Unité de Chimie Physique Théorique et Structurale (UCPTS), Rue de Bruxelles 61, B-5000 Namur, Belgium.
  • 5 University of Liege, Pneumology Laboratory, I3 Group, GIGA Research Center, B-4000 Liège, Belgium.
  • 6 Division of Respirology, Department of Pediatrics, University Hospital Liège, B-4000 Liège, Belgium.
  • 7 University of Liege, GIGA-Cancer, Laboratory of Mitochondria and Cell Communication, B34, Avenue de l'Hôpital, B-4000 Liège, Belgium.
  • 8 Universitat Pompeu Fabra (UPF), Barcelona 08002, Spain.
  • 9 ICREA, Pg. Lluis Companys 23, Barcelona 08010, Spain.
  • 10 Laboratory of Neurovascular Signaling, Department of Molecular Biology, ULB Neuroscience Institute, Université libre de Bruxelles, B-6041 Gosselies, Belgium.
PMID: 38661008 DOI: 10.1242/jcs.261744
Abstract

DPF3, along with other subunits, is a well-known component of the BAF chromatin remodeling complex, which plays a key role in regulating chromatin remodeling activity and gene expression. Here, we elucidated a non-canonical localization and role for DPF3. We showed that DPF3 dynamically localizes to the centriolar satellites in interphase and to the centrosome, spindle midzone and bridging fiber area, and midbodies during mitosis. Loss of DPF3 causes kinetochore fiber instability, unstable kinetochore-microtubule attachment and defects in chromosome alignment, resulting in altered mitotic progression, cell death and genomic instability. In addition, we also demonstrated that DPF3 localizes to centriolar satellites at the base of primary cilia and is required for ciliogenesis by regulating axoneme extension. Taken together, these findings uncover a moonlighting dual function for DPF3 during mitosis and ciliogenesis.

Keywords

Bridging fiber; Centriolar satellites; Chromosome alignment; Ciliogenesis; DPF3; Genomic instability; Mitosis.

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