2. Academic Validation
  3. The Effect of Inhibiting the Wingless/Integrated (WNT) Signaling Pathway on the Early Embryonic Disc Cell Culture in Chickens

The Effect of Inhibiting the Wingless/Integrated (WNT) Signaling Pathway on the Early Embryonic Disc Cell Culture in Chickens

  • Animals (Basel). 2024 May 4;14(9):1382. doi: 10.3390/ani14091382.
Wenjie Ren 1 2,  Dan Zheng 1 2,  Guangzheng Liu 1 2,  Gaoyuan Wu 1 2,  Yixiu Peng 1 2,  Jun Wu 1 2,  Kai Jin 1,  Qisheng Zuo 1 2,  Yani Zhang 1 2,  Guohui Li 3,  Wei Han 3,  Xiang-Shun Cui 4,  Guohong Chen 1 2,  Bichun Li 1 2,  Ying-Jie Niu 1
Affiliations

Affiliations

  • 1 Joint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education of China, Yangzhou University, Yangzhou 225009, China.
  • 2 Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.
  • 3 Poultry Institute, Chinese Academy of Agricultural Sciences, Yangzhou 225125, China.
  • 4 Department of Animal Science, Chungbuk National University, Cheongju 28644, Republic of Korea.
PMID: 38731386 DOI: 10.3390/ani14091382
Abstract

The utilization of chicken embryonic-derived pluripotent stem cell (PSC) lines is crucial in various fields, including growth and development, vaccine and protein production, and germplasm resource protection. However, the research foundation for chicken PSCs is relatively weak, and there are still challenges in establishing a stable and efficient PSC culture system. Therefore, this study aims to investigate the effects of the FGF2/ERK and Wnt/β-catenin signaling pathways, as well as different feeder layers, on the derivation and maintenance of chicken embryonic-derived PSCs. The results of this study demonstrate that the use of STO cells as feeder layers, along with the addition of FGF2, IWR-1, and XAV-939 (FIX), allows for the efficient derivation of chicken PSC-like cells. Under the FIX culture conditions, chicken PSCs express key pluripotency genes, such as POUV, SOX2, and NANOG, as well as specific proteins SSEA-1, c-Kit, and SOX2, indicating their pluripotent nature. Additionally, the embryoid body experiment confirms that these PSC-like cells can differentiate into cells of three germ layers in vitro, highlighting their potential for multilineage differentiation. Furthermore, this study reveals that chicken Eyal-Giladi and Kochav stage X blastodermal cells express genes related to the primed state of PSCs, and the FIX culture system established in this research maintains the expression of these genes in vitro. These findings contribute significantly to the understanding and optimization of chicken PSC culture conditions and provide a foundation for further exploration of the biomedical research and biotechnological applications of chicken PSCs.

Keywords

PSC; chicken; derivation; small molecules.

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