2. Stem Cell/Wnt
  3. Organoid Wnt
  4. IWR-1

IWR-1  (Synonyms: endo-IWR 1; IWR-1-endo)

Cat. No.: HY-12238 Purity: 99.49%
COA Handling Instructions

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IWR-1 is a tankyrase inhibitor which inhibits Wnt/β-catenin signaling pathway.

For research use only. We do not sell to patients.

IWR-1 Chemical Structure

IWR-1 Chemical Structure

CAS No. : 1127442-82-3

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 73 In-stock
Solution
10 mM * 1 mL in DMSO USD 73 In-stock
Solid
5 mg USD 66 In-stock
10 mg USD 118 In-stock
25 mg USD 260 In-stock
50 mg USD 455 In-stock
100 mg USD 690 In-stock
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Customer Review

Based on 36 publication(s) in Google Scholar

IWR-1 Related Antibodies

Top Publications Citing Use of Products

34 Publications Citing Use of MCE IWR-1

WB
IF

    IWR-1 purchased from MedChemExpress. Usage Cited in: Oncol Rep. 2018 Aug;40(2):877-886.  [Abstract]

    IWR-1 induces cell differentiation. (A) NB4 and (B) HL-60 cells are treated with 0 (DMSO), 5 and 10 μM IWR-1 for three days, and the expression of β-catenin and differentiation marker CD11b is assessed by western blotting.

    IWR-1 purchased from MedChemExpress. Usage Cited in: Cell Biol Int. 2017 May;41(5):534-543.  [Abstract]

    SCAPs are cultured with modified neural differentiation medium for the indicated time periods. The neuron-like cells in control group, SFRP2 over-expressed group and 10 uM IWR1-endo treated group are positive for βIII-tubulin (red) expression by using immunofluorescence staining.

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    IWR-1 is a tankyrase inhibitor which inhibits Wnt/β-catenin signaling pathway.

    IC50 & Target

    IC50: 180 nM (Wnt)
    In Vitro

    Both IWR-1 and XAV939 act as reversible Wnt pathway inhibitors and exhibit similar pharmacological effects in vitro. IWR-1 exerts its effect via interaction with Axin, while XAV939 binds TNKS directly[1]. IWR-1 (10 μM) induces stabilization of β-catenin disruption complex. IWR-1 (10 μM) is added to the medium together with MIF, the size of cell colonies is extremely decreased, and that indicates the promoting effect of MIF on NSPC proliferation is inhibited by IWR-1 in any MIF concentration group. 2, 5 and 10 μM of IWR-1 significantly inhibits the proliferation of NSPC dose-dependently. IWR-1 inhibites the promoting effect of MIF on NSPC differentiation to neuron lineage[2]. IWR-1 treatment in the presence of maximal stimulatory dose of FSH (0.5 ng/mL) results in a dose dependent inhibition of the stimulatory effect of FSH with > 75% inhibition observed at the maximal inhibitory dose of IWR-1 (1 µM). IWR-1 treatment partially reverses the FSH-induced inhibition of granulosa cell CARTPT mRNA expression[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    IWR-1 Related Antibodies
    Molecular Weight

    409.44

    Formula

    C25H19N3O3
    CAS No.
    Appearance

    Solid

    Color

    Off-white to yellow

    SMILES

    O=C(NC1=C2N=CC=CC2=CC=C1)C3=CC=C(N(C([C@]4([H])[C@](C5)([H])C=C[C@]5([H])[C@]64[H])=O)C6=O)C=C3
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    Solvent & Solubility
    In Vitro: 

    DMSO : 50 mg/mL (122.12 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.4424 mL 12.2118 mL 24.4236 mL
    5 mM 0.4885 mL 2.4424 mL 4.8847 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (6.11 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (6.11 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.49%

    COA (252 KB) LCMS (267 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.4424 mL 12.2118 mL 24.4236 mL 61.0590 mL
    5 mM 0.4885 mL 2.4424 mL 4.8847 mL 12.2118 mL
    10 mM 0.2442 mL 1.2212 mL 2.4424 mL 6.1059 mL
    15 mM 0.1628 mL 0.8141 mL 1.6282 mL 4.0706 mL
    20 mM 0.1221 mL 0.6106 mL 1.2212 mL 3.0530 mL
    25 mM 0.0977 mL 0.4885 mL 0.9769 mL 2.4424 mL
    30 mM 0.0814 mL 0.4071 mL 0.8141 mL 2.0353 mL
    40 mM 0.0611 mL 0.3053 mL 0.6106 mL 1.5265 mL
    50 mM 0.0488 mL 0.2442 mL 0.4885 mL 1.2212 mL
    60 mM 0.0407 mL 0.2035 mL 0.4071 mL 1.0177 mL
    80 mM 0.0305 mL 0.1526 mL 0.3053 mL 0.7632 mL
    100 mM 0.0244 mL 0.1221 mL 0.2442 mL 0.6106 mL
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    IWR-1 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    IWR-11127442-82-3endo-IWR 1 IWR-1-endoIWR1IWR 1OrganoidWntInhibitorinhibitorinhibit

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