2. Academic Validation
  3. TBC1D10B promotes tumor progression in colon cancer via PAK4‑mediated promotion of the PI3K/AKT/mTOR pathway

TBC1D10B promotes tumor progression in colon cancer via PAK4‑mediated promotion of the PI3K/AKT/mTOR pathway

  • Apoptosis. 2024 Jun 2. doi: 10.1007/s10495-024-01972-3.
Xiao-Jv Chi # 1,  Yi-Bei Song # 1,  Haoran Zhang # 2,  Li-Qiang Wei 1,  Yong Gao 1,  Xue-Jing Miao 1,  Shu-Ting Yang 1,  Chun-Yu Lin 1,  Dong Lan 3,  Xiquan Zhang 4
Affiliations

Affiliations

  • 1 Department of Clinical Laboratory, First Affiliated Hospital of Guangxi Medical University, Key Laboratory of Clinical Laboratory Medicine of Guangxi Department of Education, Guangxi Zhuang Autonomous Region, 6 Shuangyong Road, Nanning, 530021, China.
  • 2 Department of Gastrointestinal Surgery, First Affiliated Hospital of Jinan University, 613 Huangpu Avenue West, Guangzhou, 510632, China.
  • 3 Department of Medical Oncology, First Affiliated Hospital of Guangxi Medical University, Guangxi Zhuang Autonomous Region, 6 Shuangyong Road, Nanning, 530021, China. landong@stu.gxmu.edu.cn.
  • 4 Department of Oncology, Jiangxi provincial People's Hospital, The First Affiliated Hospital of Nanchang Medical College, Nanchang, 330006, China. Zhangxiquan1243@126.com.
  • # Contributed equally.
PMID: 38824479 DOI: 10.1007/s10495-024-01972-3
Abstract

This study aimed to explore the expression, function, and mechanisms of TBC1D10B in colon Cancer, as well as its potential applications in the diagnosis and treatment of the disease.The expression levels of TBC1D10B in colon Cancer were assessed by analyzing the TCGA and CCLE databases. Immunohistochemistry analysis was conducted using tumor and adjacent non-tumor tissues from 68 colon Cancer patients. Lentiviral Infection techniques were employed to silence and overexpress TBC1D10B in colon Cancer cells. The effects on cell proliferation, migration, and invasion were evaluated using CCK-8, EDU, wound healing, and Transwell invasion assays. Additionally, GSEA enrichment analysis was used to explore the association of TBC1D10B with biological pathways related to colon Cancer. TBC1D10B was significantly upregulated in colon Cancer and closely associated with patient prognosis. Silencing of TBC1D10B notably inhibited proliferation, migration, and invasion of colon Cancer cells and promoted Apoptosis. Conversely, overexpression of TBC1D10B enhanced these cellular functions. GSEA analysis revealed that TBC1D10B is enriched in the Akt/PI3K/mTOR signaling pathway and highly correlated with PAK4. The high expression of TBC1D10B in colon Cancer is associated with poor prognosis. It influences Cancer progression by regulating the proliferation, migration, and invasion capabilities of colon Cancer cells, potentially acting through the Akt/PI3K/mTOR signaling pathway. These findings provide new targets and therapeutic strategies for the treatment of colon Cancer.

Keywords

Colon cancer; PI3K-Akt signaling; TBC1D10B.

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