1. PI3K/Akt/mTOR Autophagy
  2. PI3K Autophagy
  3. 740 Y-P

740 Y-P  (Synonyms: 740YPDGFR; PDGFR 740Y-P)

Cat. No.: HY-P0175 Purity: 99.96%
Handling Instructions Technical Support

740 Y-P (740YPDGFR; PDGFR 740Y-P) is a potent and cell-permeable PI3K activator. 740 Y-P readily binds GST fusion proteins containing both the N- and C- terminal SH2 domains of p85 but fails to bind GST alone.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

740 Y-P Chemical Structure

740 Y-P Chemical Structure

CAS No. : 1236188-16-1

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Customer Review

Based on 170 publication(s) in Google Scholar

Other Forms of 740 Y-P:

Top Publications Citing Use of Products

168 Publications Citing Use of MCE 740 Y-P

WB

    740 Y-P purchased from MedChemExpress. Usage Cited in: J Exp Clin Cancer Res. 2020 Jun 30;39(1):123.  [Abstract]

    Protein expression levels of p-AktSer473, p-mTORSer2448 with or without 740Y-P treatment are measured by Western blotting. Pretreatment with 740Y-P (PI3K agonist) inhibits downregulation of p-AktSer473 and p-mTORSer2448 expression induced by overexpression of BDH2.

    740 Y-P purchased from MedChemExpress. Usage Cited in: Acta Biomater. 2018 Nov;81:278-292.  [Abstract]

    PC12 cells are pretreated with 740-YP (30 μM), or SC79 (13.7 μM), or 3BDO (100 μM) for 1 h, and for the duration of GO (50 μg/mL) incubation for 24 h. LC3 turnover is detected by western blotting.
    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    740 Y-P (740YPDGFR; PDGFR 740Y-P) is a potent and cell-permeable PI3K activator. 740 Y-P readily binds GST fusion proteins containing both the N- and C- terminal SH2 domains of p85 but fails to bind GST alone[1].

    IC50 & Target[1]

    PI3K

     

    In Vitro

    740 Y-P (50 μg/mL; 48 hours) specificly stimulates mitogenesis in medium is better than EGF or FGF at stimulating entry into S-phase, it shows the percentage of cells in S-phase for 48.3% in C2 cells. Additionally, LY294002 (HY-10108) or Wortmannin (HY-10197) potently inhibits the mitogenic response stimulated by the peptide[1].
    740 Y-P (1 μg/mL) stimulates mitogenesis at the lowest concentration tested. The peptide stimulates mitogenesis in both the presence and absence of serum (0.5%), and in the former instance a maximal response observed at 50 μg/mL. 740Y-P to stimulate mitogenesis is highly specific and not a general feature of a cell permeable SH2 domain binding peptides[1].
    740 Y-P (30 μM; 24 hours) remarkably inhibits the level of LC3-II/LC3-I in GO-induced PC12 cells[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Western Blot Analysis[2]

    Cell Line: PC12 cells
    Concentration: 30 μM
    Incubation Time: 24 hours
    Result: Inhibited the protein expression of LC3-II.
    In Vivo

    740 Y-P is not only internalised in living cells but can also interact with p85 in vivo[1].
    740 Y-P (intraperitoneal injection; 10 mg/kg; 6 weeks) decreases the degree of ROS levels in Aβ(25-32) treated hippocampal tissues and increases the extent of AKT and PI3K phosphorylation in alzheimer’s disease (AD) rat model[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Molecular Weight

    3270.70

    Formula

    C141H222N43O39PS3

    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    Sequence

    Arg-Gln-Ile-Lys-Ile-Trp-Phe-Gln-Asn-Arg-Arg-Met-Lys-Trp-Lys-Lys-Ser-Asp-Gly-Gly-{Tyr(PO2)}-Met-Asp-Met-Ser

    Sequence Shortening

    RQIKIWFQNRRMKWKKSDGG-{Tyr(PO2)}-MDMS

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage

    Sealed storage, away from moisture and light

    Powder -80°C 2 years
    -20°C 1 year

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

    Solvent & Solubility
    In Vitro: 

    H2O : ≥ 50 mg/mL (15.29 mM)

    H2O : 20 mg/mL (6.11 mM; Need ultrasonic)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 0.3057 mL 1.5287 mL 3.0574 mL
    5 mM 0.0611 mL 0.3057 mL 0.6115 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

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    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.96%

    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O / H2O 1 mM 0.3057 mL 1.5287 mL 3.0574 mL 7.6436 mL
    5 mM 0.0611 mL 0.3057 mL 0.6115 mL 1.5287 mL
    H2O 10 mM 0.0306 mL 0.1529 mL 0.3057 mL 0.7644 mL
    15 mM 0.0204 mL 0.1019 mL 0.2038 mL 0.5096 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    Product Name:
    740 Y-P
    Cat. No.:
    HY-P0175
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