2. Academic Validation
  3. The walnut-derived peptide TW-7 improves mouse parthenogenetic embryo development of vitrified MII oocytes potentially by promoting histone lactylation

The walnut-derived peptide TW-7 improves mouse parthenogenetic embryo development of vitrified MII oocytes potentially by promoting histone lactylation

  • J Anim Sci Biotechnol. 2024 Jun 11;15(1):86. doi: 10.1186/s40104-024-01045-0.
Yaozong Wei # 1,  Bo Pan # 1,  Jianpeng Qin 1,  Beijia Cao 1,  Tianyi Lv 1,  Jiangfeng Ye 1,  Ao Ning 1,  Kunlin Du 1,  Xiangyi Chen 1,  Shuqi Zou 1,  Shengqin Zang 1,  Guozhi Yu 2,  Tianzeng Song 3,  Qiuxia Liang 4,  Guangbin Zhou 5
Affiliations

Affiliations

  • 1 State Key Laboratory of Swine and Poultry Breeding Industry, Key Laboratory of Livestock and Poultry Multi-Omics, Ministry of Agriculture and Rural Affairs, and Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China.
  • 2 College of Life Science, Sichuan Agricultural University, Ya'an, 625014, Sichuan, China.
  • 3 Institute of Animal Science, Xizang Academy of Agricultural and Animal Husbandry Science, Lhasa, 850009, Xizang, China.
  • 4 College of Life Science, Sichuan Agricultural University, Ya'an, 625014, Sichuan, China. LiangQX@sicau.edu.cn.
  • 5 State Key Laboratory of Swine and Poultry Breeding Industry, Key Laboratory of Livestock and Poultry Multi-Omics, Ministry of Agriculture and Rural Affairs, and Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China. zguangbin@sicau.edu.cn.
  • # Contributed equally.
PMID: 38858724 DOI: 10.1186/s40104-024-01045-0
Abstract

Background: Previous studies have shown that the vitrification of metaphase II (MII) oocytes significantly represses their developmental potential. Abnormally increased oxidative stress is the probable factor; however, the underlying mechanism remains unclear. The walnut-derived peptide TW-7 was initially isolated and purified from walnut protein hydrolysate. Accumulating evidences implied that TW-7 was a powerful antioxidant, while its prospective application in oocyte cryopreservation has not been reported.

Result: Here, we found that parthenogenetic activation (PA) zygotes derived from vitrified MII oocytes showed elevated ROS level and delayed progression of pronucleus formation. Addition of 25 μmol/L TW-7 in warming, recovery, PA, and embryo culture medium could alleviate oxidative stress in PA zygotes from vitrified mouse MII oocytes, furtherly increase proteins related to histone lactylation such as LDHA, LDHB, and EP300 and finally improve histone lactylation in PA zygotes. The elevated histone lactylation facilitated the expression of minor zygotic genome activation (ZGA) genes and preimplantation embryo development.

Conclusions: Our findings revealed the mechanism of oxidative stress inducing repressed development of PA embryos from vitrified mouse MII oocytes and found a potent and easy-obtained short peptide that could significantly rescue the decreased developmental potential of vitrified oocytes, which would potentially contribute to reproductive medicine, animal protection, and breeding.

Keywords

Histone lactylation; Oocyte; TW-7; Vitrification; Zygotic genome activation.

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