2. Academic Validation
  3. Fabrication and Evaluation of Hyaluronidase-Responsive Scaffolds by Electrospinning with Antibacterial Properties for Tympanic Membrane Repair

Fabrication and Evaluation of Hyaluronidase-Responsive Scaffolds by Electrospinning with Antibacterial Properties for Tympanic Membrane Repair

  • ACS Biomater Sci Eng. 2024 Jul 8;10(7):4400-4410. doi: 10.1021/acsbiomaterials.4c00060.
Zhechen Yuan 1 2,  Bing Mei Teh 3,  Xiaoling Liu 4,  Ziqian Liu 4,  Juntao Huang 5,  Yi Hu 5,  Chengchen Guo 6,  Yi Shen 5
Affiliations

Affiliations

  • 1 Department of Otolaryngology Head and Neck Surgery, Ningbo No.2 Hospital, Ningbo 315010, China.
  • 2 Health Science Center, Ningbo University, Ningbo 315211, Zhejiang, China.
  • 3 Department of Otolaryngology Head and Neck Surgery, Monash Health, Faculty of Medicine, Nursing and Health Sciences, Monash University, Clayton 3800, Victoria, Australia.
  • 4 Department of Mechanical, Materials and Manufacturing Engineering, The University of Nottingham Ningbo China, Ningbo 315100, China.
  • 5 Department of Otolaryngology Head and Neck Surgery, Ningbo Medical Center Lihuili Hospital, The Affiliated Lihuili Hospital of Ningbo University, Ningbo 315040, China.
  • 6 School of Engineering, Westlake University, Hangzhou 310030, China.
PMID: 38917429 DOI: 10.1021/acsbiomaterials.4c00060
Abstract

Tympanic membrane perforation (TMP) is prevalent in clinical settings. Patients with TMPs often suffer from infections caused by Staphylococcus aureus and Pseudomonas aeruginosa, leading to middle ear and external ear canal infections, which hinder eardrum healing. The objective of this study is to fabricate an enzyme-responsive Antibacterial electrospun scaffold using poly(lactic-co-glycolic acid) and hyaluronic acid for the treatment of infected TMPs. The properties of the scaffold were characterized, including morphology, wettability, mechanical properties, degradation properties, antimicrobial properties, and biocompatibility. The results indicated that the fabricated scaffold had a core-shell structure and exhibited excellent mechanical properties, hydrophobicity, degradability, and cytocompatibility. Furthermore, in vitro Bacterial tests and ex vivo investigations on eardrum infections suggested that this scaffold possesses hyaluronidase-responsive Antibacterial properties. It may rapidly release Antibiotics when exposed to the Enzyme released by S. aureus and P. aeruginosa. These findings suggest that the scaffold has great potential for repairing TMPs with infections.

Keywords

antibacterial; electrospinning; hyaluronidase-responsive; repair; tissue engineering; tympanic membrane perforation.

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