2. Academic Validation
  3. Death associated protein like 1 acts as a novel tumor suppressor in melanoma by increasing the stability of P21 protein

Death associated protein like 1 acts as a novel tumor suppressor in melanoma by increasing the stability of P21 protein

  • Mol Cell Biochem. 2024 Jul 9. doi: 10.1007/s11010-024-05067-0.
Xiaoyan Liu # 1,  Xiaojuan Hu # 1,  Meiyu Jing 1,  Lijin Huang 1,  Yaqi You 1,  Yaru Zhang 2,  Ke Li 2,  Yunhai Tu 3,  Youjia Liu 1,  Xiaogang Chen 4,  Jianzhong Su 2,  J Fielding Hejtmancik 5,  Ling Hou 1,  Xiaoyin Ma 6
Affiliations

Affiliations

  • 1 Laboratory of Developmental Cell Biology and Disease, State Key Laboratory of Ophthalmology, Optometry and Visual Science, Eye Hospital, Wenzhou Medical University, Wenzhou, 325027, China.
  • 2 National Engineering Research Center of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical University, Wenzhou, 325027, China.
  • 3 National Clinical Research Center for Ocular Diseases, Eye Hospital, Wenzhou Medical University, Wenzhou, 325027, China.
  • 4 State Key Laboratory of Ophthalmology, Optometry and Visual Science, Eye Hospital, Wenzhou Medical University, Wenzhou, 325027, China.
  • 5 Ophthalmic Genetics and Visual Function Branch, National Eye Institute, National Institutes of Health, Bethesda, MD, 20892, USA.
  • 6 Laboratory of Developmental Cell Biology and Disease, State Key Laboratory of Ophthalmology, Optometry and Visual Science, Eye Hospital, Wenzhou Medical University, Wenzhou, 325027, China. xyma2015@wmu.edu.cn.
  • # Contributed equally.
PMID: 38980592 DOI: 10.1007/s11010-024-05067-0
Abstract

Melanoma is a primary malignant tumor with high lethality, which occurs in the skin and eye tissues, while the molecular mechanisms of melanomagenesis remain largely unknown. Here, we show that death-associated protein-like 1 (DAPL1) expression is lower in melanoma tissues than in paracancerous tissues or nevus tissues, and Uveal melanoma patients with lower DAPL1 expression have a poorer survival rate than those with higher expression of DAPL1. Overexpression of DAPL1 inhibits proliferation of cultured melanoma cells, whereas knockdown of DAPL1 increases cell proliferation. Tumor transplantation experiment results also demonstrate that DAPL1 inhibits tumorigenesis of melanoma cells both in subretinal and subcutaneous tissues of nude mice in vivo. Finally, DAPL1 inhibits proliferation of melanoma cells by increasing the protein level of P21 via decreasing the ubiquitin mediated degradation of P21 and promoting its stability. Conversely, knockdown of P21 neutralizes the effects of inhibition of DAPL1 on melanoma cell proliferation and enhances the severity of melanoma tumorigenesis. These results suggest that DAPL1 is a novel melanoma tumor suppressor gene and thus a potential therapeutic target for melanoma.

Keywords

Cell proliferation; DAPL1; Melanoma; P21; Protein stability.

Figures
Products