2. Academic Validation
  3. Dehydroevodiamine inhibits PEDV through regulateing ERK1/2 MAPK pathway in Vero cells

Dehydroevodiamine inhibits PEDV through regulateing ERK1/2 MAPK pathway in Vero cells

  • Virology. 2024 Jul 2:598:110166. doi: 10.1016/j.virol.2024.110166.
Kaiyuan Li 1,  Huiru Wang 1,  Tingting Chen 1,  Xuebo Wang 2,  Xue Wang 1,  Muqi Zhong 3,  Xueyan Gao 1,  Zhihui Hao 4
Affiliations

Affiliations

  • 1 College of Veterinary Medicine, China Agricultural University, Beijing, 100193, China; Key Biology Laboratory of Chinese Veterinary Medicine, Ministry of Agriculture and Rural Affairs, Beijing, 100193, China.
  • 2 Shandong Lvdu Bio-sciences & Technology Co., LTD, Binzhou, 256600, China.
  • 3 College of Chemistry and Pharmacy, Qingdao Agricultural University, Qingdao, 266109, China.
  • 4 College of Veterinary Medicine, China Agricultural University, Beijing, 100193, China; Key Biology Laboratory of Chinese Veterinary Medicine, Ministry of Agriculture and Rural Affairs, Beijing, 100193, China. Electronic address: haozhihui@cau.edu.cn.
PMID: 39024722 DOI: 10.1016/j.virol.2024.110166
Abstract

Porcine epidemic diarrhea virus (PEDV) results in severe economic losses to the swine industry due to its widespread prevalence and high mortality. Currently, there is no effective treatment against PEDV. New Antiviral therapies are urgently needed to control this highly contagious pathogen. In this research, the anti-PEDV activity and mechanism of Dehydroevodiamine (DHED) were investigated in vitro. Our results showed that DHED exerted satisfactory anti-PEDV activity by ameliorating cytopathic effects (CPEs), reducing virus titer, and inhibiting PEDV N protein expression and gene transcription dose-dependently. The Antiviral mechanism of DHED is related to its inhibition of the entry, replication, and assembly stages of PEDV life cycle. In addition, DHED can regulate the MAPK signaling pathway, and suppress phosphorylated ERK1/2 activation, thus exerting Antiviral effects. In conclusion, our research confirmed the anti-PEDV activity and mechanism of DHED, preliminarily providing a new strategy for anti-PEDV drug development.

Keywords

Antiviral; Dehydroevodiamine(DHED); ERK1/2 MAPK pathway; Porcine epidemic diarrhea virus (PEDV).

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