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  3. Hfq mediates transcriptome-wide RNA structurome reprogramming under virulence-inducing conditions in a phytopathogen

Hfq mediates transcriptome-wide RNA structurome reprogramming under virulence-inducing conditions in a phytopathogen

  • Cell Rep. 2024 Aug 27;43(8):114544. doi: 10.1016/j.celrep.2024.114544.
Canfeng Hua 1,  Jiadai Huang 1,  Yue Sun 1,  Tingting Wang 1,  Youyue Li 1,  Zining Cui 2,  Xin Deng 3
Affiliations

Affiliations

  • 1 Department of Biomedical Sciences, City University of Hong Kong, Hong Kong, China.
  • 2 National Key Laboratory of Green Pesticide, Integrative Microbiology Research Center, Guangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Plant Protection, South China Agricultural University, Guangzhou, China.
  • 3 Department of Biomedical Sciences, City University of Hong Kong, Hong Kong, China; Shenzhen Research Institute, City University of Hong Kong, Shenzhen, Guangdong, China; Tung Biomedical Sciences Center, City University of Hong Kong, Hong Kong, China. Electronic address: xindeng@cityu.edu.hk.
PMID: 39052478 DOI: 10.1016/j.celrep.2024.114544
Abstract

Although RNA structures play important roles in regulating gene expression, the mechanism and function of mRNA folding in plant Bacterial pathogens remain elusive. Therefore, we perform dimethyl sulfate Sequencing (DMS-seq) on the Pseudomonas syringae under nutrition-rich and -deficient conditions, revealing that the mRNA structure changes substantially in the minimal medium (MM) that tunes global translation efficiency (TE), thereby inducing virulence. This process is led by the increased expression of hfq, which is directly activated by transcription regulators RpoS and CysB. The co-occurrence of Hfq and RpoS in diverse bacteria and the deep conservation of Hfq Y25 is critical for RNA-mediated regulation and implicates the wider biological importance of mRNA structure and feedback loops in the control of global gene expression.

Keywords

CP: Microbiology; CP: Molecular biology; CysB; DMS-seq; Hfq; Hfq(Y25D); Pseudomonas savastanoi; RpoS.

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