2. Academic Validation
  3. Mutant p53 achieves function by regulating EGR1 to induce epithelial mesenchymal transition

Mutant p53 achieves function by regulating EGR1 to induce epithelial mesenchymal transition

  • Tissue Cell. 2024 Oct:90:102510. doi: 10.1016/j.tice.2024.102510.
Weipei Meng 1,  Shilong Yu 2,  Yan Li 1,  Haichen Wang 3,  Yuqing Feng 3,  Wanyue Sun 1,  Ying Liu 1,  Shilong Sun 4,  Haifeng Liu 5
Affiliations

Affiliations

  • 1 Department of Toxicology, School of Public Health, Jilin University, Changchun, Jilin 130021, China.
  • 2 Interventional Center, Jilin Cancer Hospital, No. 1018 Huguang Rd, Chaoyang, Changchun 130012, China.
  • 3 NHC Key Laboratory of Radiobiology, School of Public Health, Jilin University, Changchun 130021, China.
  • 4 NHC Key Laboratory of Radiobiology, School of Public Health, Jilin University, Changchun 130021, China. Electronic address: slsun@jlu.edu.cn.
  • 5 Interventional Center, Jilin Cancer Hospital, No. 1018 Huguang Rd, Chaoyang, Changchun 130012, China. Electronic address: liuhaifeng529@163.com.
PMID: 39126833 DOI: 10.1016/j.tice.2024.102510
Abstract

The epithelial-mesenchymal transition (EMT) plays a crucial role in lung Cancer metastasis, rendering it a promising therapeutic target. Research has shown that non-small cell lung Cancer (NSCLC) with p53 mutations exhibits an increased tendency for Cancer metastasis. However, the exact contribution of the p53-R273H mutation to tumor metastasis remains uncertain in the current literature. Our study established the H1299-p53-R273H cell model successfully by transfecting the p53-R273H plasmid into H1299 cells. We observed that p53-R273H promotes cell proliferation, migration, invasion, and EMT through CCK-8, wound healing, transwell, western blot and immunofluorescence assays. Notably, the expression of EGR1 was increased in H1299-p53-R273H cells. Knocking out EGR1 in these cells hindered the progression of EMT. ChIP-PCR experiments revealed that p53-R273H binds to the EGR1 promoter sequence, thereby regulating its expression. These findings suggest that p53-R273H triggers EMT by activating EGR1, thereby offering a potential therapeutic approach for lung Cancer treatment.

Keywords

EGR1; EMT; NSCLC; P53-R273H.

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