Tert-butylhydroquinone promotes skin flap survival by inhibiting oxidative stress mediated by the Nrf2/HO-1 signalling pathway

Background and purpose: Skin flaps are among the most important means of wound repair in clinical settings. However, partial or even total distal necrosis may occur after a FLAP operation, with severe consequences for both patients and doctors. This study investigated whether tert-butylhydroquinone (TBHQ), a known agonist of the transcription factor nuclear factor erythroid 2-related factor 2 (Nrf2), and an antioxidant, could promote skin FLAP survival.

Experimental approach: McFarlane skin FLAP models were established in male Sprague-Dawley rats and then randomly divided into control, low-dose TBHQ, and high-dose TBHQ treatment groups. On postoperative day 7, the survival and blood flow of the skin flaps were assessed. Using FLAP tissue samples, angiogenesis, inflammation, Apoptosis, Autophagy, and Nrf2/haem oxygenase 1 (HO-1) signalling pathway activity were measured with immunohistochemical techniques and western blotting.

Key results: TBHQ dose-dependently stimulated the Nrf2/HO-1 signalling pathway, inducing Autophagy through the up-regulation of LC3B and beclin 1 and concurrently suppressing p62 expression. Additionally, TBHQ hindered Apoptosis by enhancing Bcl-2 expression while inhibiting the expression of Bax. It suppressed inflammation by inhibiting the expression of interleukin 1β, interleukin 6, and tumour necrosis factor-α and enhanced angiogenesis by promoting the expression of vascular endothelial growth factor.

Conclusion and implications: In summary, TBHQ promoted FLAP survival in rats by up-regulating the Nrf2/HO-1 signalling pathway. As TBHQ is already widely used as a food additive, it could offer an acceptable means of improving clinical outcomes following skin FLAP surgery in patients.

Nrf2/HO‐1; apoptosis; autophagy; tert‐butylhydroquinone.