2. Academic Validation
  3. Betulonic Acid Inhibits Type-2 Porcine Reproductive and Respiratory Syndrome Virus Replication by Downregulating Cellular ATP Production

Betulonic Acid Inhibits Type-2 Porcine Reproductive and Respiratory Syndrome Virus Replication by Downregulating Cellular ATP Production

  • Int J Mol Sci. 2024 Sep 26;25(19):10366. doi: 10.3390/ijms251910366.
Feixiang Long 1 2,  Lizhan Su 1 2,  Mingxin Zhang 1 2,  Shuhua Wang 1 2,  Qian Sun 1 2,  Jinyi Liu 1 2,  Weisan Chen 3,  Haihong Wang 4,  Jianxin Chen 1 2
Affiliations

Affiliations

  • 1 Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, Guangzhou 510642, China.
  • 2 College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
  • 3 Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, VIC 3086, Australia.
  • 4 College of Life Sciences, South China Agricultural University, Guangzhou 510642, China.
PMID: 39408695 DOI: 10.3390/ijms251910366
Abstract

Porcine reproductive and respiratory syndrome (PRRS), caused by PRRS virus (PRRSV) Infection, has been a serious threat to the pork industry worldwide and continues to bring significant economic loss. Current vaccination strategies offer limited protection against PRRSV transmission, highlighting the urgent need for novel Antiviral approaches. In the present study, we reported for the first time that betulonic acid (BA), a widely available Pentacyclic Triterpenoids throughout the plant kingdom, exhibited potent inhibition on PRRSV infections in both Marc-145 cells and primary porcine alveolar macrophages (PAMs), with IC50 values ranging from 3.3 µM to 3.7 µM against three different type-2 PRRSV strains. Mechanistically, we showed that PRRSV replication relies on energy supply from cellular ATP production, and BA inhibits PRRSV Infection by reducing cellular ATP production. Our findings indicate that controlling host ATP production could be a potential strategy to combat PRRSV infections, and that BA might be a promising therapeutic agent against PRRSV epidemics.

Keywords

adenosine triphosphate (ATP); anti-PRRSV activity; betulonic acid (BA); porcine reproductive and respiratory syndrome virus (PRRSV).

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