2. Metabolic Enzyme/Protease
  3. Endogenous Metabolite
  4. ATP

ATP  (Synonyms: Adenosine 5'-triphosphate)

Cat. No.: HY-B2176 Purity: 98.83%
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ATP (Adenosine 5'-triphosphate) is a central component of energy storage and metabolism in vivo. ATP provides the metabolic energy to drive metabolic pumps and serves as a coenzyme in cells. ATP is an important endogenous signaling molecule in immunity and inflammation.

For research use only. We do not sell to patients.

ATP Chemical Structure

ATP Chemical Structure

CAS No. : 56-65-5

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500 mg USD 96 In-stock
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Based on 71 publication(s) in Google Scholar

Other Forms of ATP:

ATP Related Antibodies

Top Publications Citing Use of Products

70 Publications Citing Use of MCE ATP

WB

    ATP purchased from MedChemExpress. Usage Cited in: Transl Res. 2022.

    LPS/ATP treatment significantly promotes expression of NLRP3 and production of N-GSDMD in HAEC. HAEC are pretreated with LPS (500ng/ml) for 5 h and then stimulated with ATP (5 mM) for 1 h.

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    ATP (Adenosine 5'-triphosphate) is a central component of energy storage and metabolism in vivo. ATP provides the metabolic energy to drive metabolic pumps and serves as a coenzyme in cells. ATP is an important endogenous signaling molecule in immunity and inflammation[1][2].

    IC50 & Target

    Human Endogenous Metabolite

     

    In Vitro

    ATP (5 mM; 1 hour) co-treatment with LPS (1 μg/mL) has a synergistic effect on the activation of the NLRP3 inflammasome in HGFs[3].
    ATP (2 mM; 0.5-24 hours) induces secretion of IL-1β, KC and MIP-2 from BMDMs in a caspase-1 activation-dependent manner[4].
    ATP promotes neutrophil chemotaxis in vitro[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    ATP Related Antibodies
    In Vivo

    ATP (50 mg/kg; i.p.) protects mice against bacterial infection in vivo[4].
    ATP induces the secretion of IL-1β, KC and MIP-2 and neutrophils recruitment in vivo[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Four-week-old Kunming mice (18-22 g)[4]
    Dosage: 50 mg/kg
    Administration: Intraperitoneal injection, before bacterial (E. coli) challenge
    Result: Protected mice from bacterial infection.
    Molecular Weight

    507.18

    Formula

    C10H16N5O13P3
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=P(O)(O)OP(OP(OC[C@H]1O[C@@H](N2C3=NC=NC(N)=C3N=C2)[C@H](O)[C@@H]1O)(O)=O)(O)=O
    Structure Classification
    Initial Source

    widespread
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    -20°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    H2O : ≥ 100 mg/mL (197.17 mM)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.9717 mL 9.8584 mL 19.7169 mL
    5 mM 0.3943 mL 1.9717 mL 3.9434 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
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    Concentration
    ×
    Volume
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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 100 mg/mL (197.17 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 98.92%

    COA (251 KB) HNMR (275 KB) CNMR (320 KB) RP-HPLC (274 KB) LCMS (95 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O 1 mM 1.9717 mL 9.8584 mL 19.7169 mL 49.2922 mL
    5 mM 0.3943 mL 1.9717 mL 3.9434 mL 9.8584 mL
    10 mM 0.1972 mL 0.9858 mL 1.9717 mL 4.9292 mL
    15 mM 0.1314 mL 0.6572 mL 1.3145 mL 3.2861 mL
    20 mM 0.0986 mL 0.4929 mL 0.9858 mL 2.4646 mL
    25 mM 0.0789 mL 0.3943 mL 0.7887 mL 1.9717 mL
    30 mM 0.0657 mL 0.3286 mL 0.6572 mL 1.6431 mL
    40 mM 0.0493 mL 0.2465 mL 0.4929 mL 1.2323 mL
    50 mM 0.0394 mL 0.1972 mL 0.3943 mL 0.9858 mL
    60 mM 0.0329 mL 0.1643 mL 0.3286 mL 0.8215 mL
    80 mM 0.0246 mL 0.1232 mL 0.2465 mL 0.6162 mL
    100 mM 0.0197 mL 0.0986 mL 0.1972 mL 0.4929 mL
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    ATP Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    ATP56-65-5Adenosine 5'-triphosphateEndogenous MetaboliteenergystoragemetabolismimmunityinflammationNLRP3inflammasomecytokineInhibitorinhibitorinhibit

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