2. Academic Validation
  3. Novel Ser74 of NF-κB/IκBα phosphorylated by MAPK/ERK regulates temperature adaptation in oysters

Novel Ser74 of NF-κB/IκBα phosphorylated by MAPK/ERK regulates temperature adaptation in oysters

  • Cell Commun Signal. 2024 Nov 11;22(1):539. doi: 10.1186/s12964-024-01923-0.
Chaogang Wang # 1 2 3,  Zhuxiang Jiang # 2 3 4,  Mingyang Du 2 3 4,  Rihao Cong 5 2 3 6,  Wei Wang 2 7 3,  Taiping Zhang 2 3 4,  Jincheng Chen 2 3 4,  Guofan Zhang 1 5 2 3 8 6,  Li Li 9 10 11 12 13 14
Affiliations

Affiliations

  • 1 Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture(CAS), Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China.
  • 2 Shandong Province Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China.
  • 3 National and Local Joint Engineering Laboratory of Ecological Mariculture, Qingdao, China.
  • 4 University of Chinese Academy of Sciences, Beijing, China.
  • 5 Laboratory for Marine Biology and Biotechnology, Qingdao Marine Science and Technology Center, Qingdao, China.
  • 6 Southern Marine Science and Engineering Guangdong Laboratory (Zhanjiang), Zhanjiang, China.
  • 7 Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao Marine Science and Technology Center, Qingdao, China.
  • 8 Shandong Technology Innovation Center of Oyster Seed Industry, Qingdao, China.
  • 9 Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture(CAS), Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China. lili@qdio.ac.cn.
  • 10 Shandong Province Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China. lili@qdio.ac.cn.
  • 11 Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao Marine Science and Technology Center, Qingdao, China. lili@qdio.ac.cn.
  • 12 National and Local Joint Engineering Laboratory of Ecological Mariculture, Qingdao, China. lili@qdio.ac.cn.
  • 13 University of Chinese Academy of Sciences, Beijing, China. lili@qdio.ac.cn.
  • 14 Shandong Technology Innovation Center of Oyster Seed Industry, Qingdao, China. lili@qdio.ac.cn.
  • # Contributed equally.
PMID: 39529137 DOI: 10.1186/s12964-024-01923-0
Abstract

Phosphorylation of Ser32 and Ser36 controls the degradation of IκBα is the conserved cascade mechanisms of immune core signaling pathway, NF-κB pathway in metazoans, but it's response to abiotic stress and the presence of novel phosphorylation mechanisms in Other species remain unclear. Herein, we reported a novel heat-induced phosphorylation site (Ser74) at oysters' major IκBα, which independently regulated ubiquitination-proteasome degradation without the requirement of phosphorylation at S32 and S36. And this site was phosphorylated by ERK/MAPK pathway, which then promoted REL nuclear translocation to activate cell survival related genes to defend heat-stress. The MAPK-NF-κB cascade exhibited divergent thermal responses and adaptation patterns between two congeneric oyster species with differential habitat temperatures, indicating its involvement in shaping temperature adaptation. This study demonstrated that the existence of complex and unique phosphorylation-mediated signaling transduction mechanism in marine invertebrates, and expanded our understanding of the evolution and function of established classical pathway crosstalk mechanisms.

Keywords

ERK; IκBα; MAPK pathway; NF-κB pathway; Oysters; Temperature Adaptation.

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