2. Academic Validation
  3. Methionine deprivation inhibits glioma proliferation and EMT via the TP53TG1/miR-96-5p/STK17B ceRNA pathway

Methionine deprivation inhibits glioma proliferation and EMT via the TP53TG1/miR-96-5p/STK17B ceRNA pathway

  • NPJ Precis Oncol. 2024 Nov 21;8(1):270. doi: 10.1038/s41698-024-00763-y.
Jiafeng Li # 1 2 3,  Ruijie Liu # 1 2 3,  Hong Hu # 1 2 3,  Yishuai Huang # 1 2 3,  Ying Shi 4,  Honglei Li 1 2 3,  Hao Chen 1 2 3,  Meng Cai 1 2 3,  Ning Wang 5,  Tao Yan 6 7,  Kaikai Wang 8,  Huailei Liu 9 10 11
Affiliations

Affiliations

  • 1 Department of Neurosurgery, First Affiliated Hospital of Harbin Medical University, Harbin, 150001, Heilongjiang Province, China.
  • 2 Key Colleges and Universities Laboratory of Neurosurgery in Heilongjiang Province, Harbin, 150001, Heilongjiang Province, China.
  • 3 Institute of Neuroscience, Sino-Russian Medical Research Center, Harbin Medical University, Harbin, 150001, Heilongjiang Province, China.
  • 4 Departments of Magnetic Resonance, The First Affiliated Hospital of Harbin Medical University, Harbin, 150001, Heilongjiang Province, China.
  • 5 Department of Critical Care Medicine, First Affiliated Hospital of Harbin Medical University, Harbin, 150001, Heilongjiang Province, China.
  • 6 Central Laboratory, Linyi People's Hospital, Linyi, 276000, Shandong Province, China.
  • 7 Linyi Key Laboratory of Neurophysiology, Linyi People's Hospital, Linyi, 276000, Shandong Province, China.
  • 8 Department of Neurosurgery, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Zhejiang Province, Hangzhou, PR China. wangkaikai@zju.edu.cn.
  • 9 Department of Neurosurgery, First Affiliated Hospital of Harbin Medical University, Harbin, 150001, Heilongjiang Province, China. liuhuaileinsdm@hrbmu.edu.cn.
  • 10 Key Colleges and Universities Laboratory of Neurosurgery in Heilongjiang Province, Harbin, 150001, Heilongjiang Province, China. liuhuaileinsdm@hrbmu.edu.cn.
  • 11 Institute of Neuroscience, Sino-Russian Medical Research Center, Harbin Medical University, Harbin, 150001, Heilongjiang Province, China. liuhuaileinsdm@hrbmu.edu.cn.
  • # Contributed equally.
PMID: 39572759 DOI: 10.1038/s41698-024-00763-y
Abstract

Recent research highlights the significant impact of methionine metabolism on glioma progression. An increasing amount of compelling evidence bridges long non-coding RNAs to abnormal metabolism in gliomas. However, the specific role of long non-coding RNAs in methionine metabolism regulating glioma progression remains unclear. This study reveals that methionine deprivation inhibits the proliferation, migration, and invasion capabilities of gliomas. Interestingly, the expression of TP53TG1, a long non-coding RNA, is also suppressed. TP53TG1 is highly expressed in gliomas and associated with poor patient outcomes. Subsequently, our data proves that inhibition of TP53TG1 suppresses glioma cell proliferation and the epithelial-mesenchymal transition process both in vitro and in vivo. Ultimately, we found that the underlying mechanism involves a competing endogenous RNA regulating network, in which TP53TG1 modulates the target protein STK17B by competitively binding to miR-96-5p, thus regulating glioma progression. These findings suggest that targeting methionine deprivation could be a promising approach for the clinical treatment of glioma.

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