2. Academic Validation
  3. Role of transglutaminase 2 in promoting biglycan synthesis in idiopathic gingival fibromatosis

Role of transglutaminase 2 in promoting biglycan synthesis in idiopathic gingival fibromatosis

  • BMC Oral Health. 2024 Nov 21;24(1):1422. doi: 10.1186/s12903-024-05211-8.
Yurika Ninomiya # 1,  Shinji Matsuda # 2,  Shigeki Suzuki 3 4,  Shizu Hirata-Tsuchiya 5,  Tomoya Ueda 1,  Fuminori Nakashima 1,  Keisuke Yasuda 1,  Shogo Shimada 1,  Takumi Memida 6,  Tetsuya Yoshimoto 7,  Satoru Yamada 3,  Kazuhisa Ouhara 1,  Noriyoshi Mizuno 1
Affiliations

Affiliations

  • 1 Department of Periodontal Medicine, Graduate School of Biomedical and Health Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima, 734-8553, Japan.
  • 2 Department of Periodontal Medicine, Graduate School of Biomedical and Health Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima, 734-8553, Japan. matsudas@hiroshima-u.ac.jp.
  • 3 Department of Periodontology and Endodontology, Tohoku University Graduate School of Dentistry, Sendai, Japan.
  • 4 Department of Operative Dentistry, Dentistry and Pharmaceutical Sciences, Okayama University Graduate School of Medicine, Sendai, Japan.
  • 5 Department of Biological Endodontics, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan.
  • 6 Department of Oral Science and Translation Research, College of Dental Medicine, Nova Southeastern University, Fort Lauderdale, USA.
  • 7 Center of Oral Clinical Examination, Hiroshima University Hospital, Hiroshima, Japan.
  • # Contributed equally.
PMID: 39574071 DOI: 10.1186/s12903-024-05211-8
Abstract

Background: This study aimed to elucidate the pathogenesis of idiopathic gingival fibromatosis (IGF).

Methods: Human gingival fibroblasts (hGFs) were isolated from patients with IGF and periodontitis. Differential gene expression in the hGFs was analyzed using RNA Sequencing. Extracellular matrix-related gene expression in the hGFs was analyzed. The effect of specific protein (SP)1 inhibitor or recombinant human transglutaminase 2 (rh-TGM2) on biglycan (BGN) expression in hGFs was also determined.

Results: RNA Sequencing showed that TGM2 expression was downregulated and BGN mRNA expression was upregulated in patients with IGF relative to periodontitis. rh-TGM2 stimulation of hGFs in patients with IGF significantly reduced BGN expression. SP1 inhibitors downregulated BGN expression in the hGFs.

Conclusion: BGN upregulation via SP1 causes TGM2 downregulation in gingival fibroblasts in IGF.

Keywords

Biglycan; Human gingival fibroblast; Idiopathic gingival fibromatosis; Periodontitis; Specific protein 1; Transglutaminase 2.

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