2. Academic Validation
  3. Mir-615-5p inhibits cervical cancer progression by targeting TMIGD2

Mir-615-5p inhibits cervical cancer progression by targeting TMIGD2

  • Hereditas. 2025 Jan 9;162(1):4. doi: 10.1186/s41065-024-00363-7.
Yan Zhao 1,  Haitao Chen 2,  Wenhui Zhang 3,  Wei Shang 4,  Jinwei Cao 5,  Huijuan Zhao 6,  Zhixiang Zou 7
Affiliations

Affiliations

  • 1 Department of Gynecology, Taiyuan Central Hospital Affiliated to Shanxi Medical University, Taiyuan, Shanxi, China.
  • 2 Department of Obstetrics and Gynecology, Zhucheng Hospital of Traditional Chinese Medicine, Weifang, 262200, Shandong, China.
  • 3 Department of Medical Imaging, The Third Hospital of Shijiazhuang, No. 15, Tiyu South Street, Chang'an District, Shijiazhuang, 050051, Hebei, China. wenhuiZhang_sjz@163.com.
  • 4 Department of Obstetrics and Gynecology, Renqiu People's Hospital, Renqiu, 062550, Hebei, China.
  • 5 Department of Medical Imaging, The Sixth Hospital of Shijiazhuang, Shijiazhuang, 050051, Hebei, China.
  • 6 Second Department of Obstetrics and Gynecology, The Sixth Hospital of Shijiazhuang, Shijiazhuang, 050051, Hebei, China.
  • 7 Obstetrics and Gynecology Medical Centre, The First Affiliated Hospital of Hunan University of Traditional Chinese Medicine, No.105, Shaoshan Middle Road, Yuhua District, Changsha, 410007, Hunan, China. Zouzhixiang_hnzyfy@163.com.
PMID: 39789663 DOI: 10.1186/s41065-024-00363-7
Abstract

Background: Cervical Cancer (CC) is a prevalent gynecological malignancy, contributing to a substantial number of fatalities among women. MicroRNAs (miRNAs) have emerged as promising biomarkers with significant potential for the early detection and prognosis of CC.

Objective: This study aimed to explore the clinical significance and biological role of miR-615-5p in CC, with the goal of identifying novel biomarkers for this disease.

Materials and methods: The levels of miR-615-5p and TMIGD2 mRNA in tissue samples and cells were quantified through quantitative reverse transcription Real-Time PCR, followed by statistical analyses to investigate the correlation between miR-615-5p and clinical data. The effects of miR-615-5p on the proliferation and metastasis of CC cells were evaluated using the Cell Counting Kit-8 and Transwell assays. The potential mechanism of miR-615-5p was elucidated by bioinformatics analyses and Dual-luciferase reporter assay. Western blotting was employed to measure the protein levels of TMIGD2.

Results: In CC, the downregulation of miR-615-5p was related to poor prognosis and emerged as an independent prognostic factor. The levels of miR-615-5p were reduced in CC cells. miR-615-5p overexpression restrained the proliferation and metastasis of CC cells. Furthermore, TMIGD2 was identified as a target gene regulated by miR-615-5p, and its expression was notably elevated in CC. The influence of miR-615-5p on the biological behaviors of CC cells was mediated through the modulation of TMIGD2.

Conclusions: Downregulation of miR-615-5p was a prognostic indicator of poor prognosis in CC. miR-615-5p exerted its tumor-suppressive effects by inhibiting cell growth and metastasis through the regulation of TMIGD2.

Keywords

Cervical cancer; Prognosis; Progression; TMIGD2; miR-615-5p.

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