2. Academic Validation
  3. PCP-B peptides and CrRLK1L receptor kinases control pollination via pH gating of aquaporins in Arabidopsis

PCP-B peptides and CrRLK1L receptor kinases control pollination via pH gating of aquaporins in Arabidopsis

  • Dev Cell. 2025 Jan 7:S1534-5807(24)00765-2. doi: 10.1016/j.devcel.2024.12.026.
Zhiwen Liu 1,  Xiaonan Chu 1,  Weiwei Ren 2,  Lijun Cheng 1,  Chen Liu 3,  Congcong Wang 1,  Sihan Gao 1,  Shaojun Dai 2,  Chao Li 4
Affiliations

Affiliations

  • 1 School of Life Sciences, East China Normal University, Shanghai 200241, China.
  • 2 Development Center of Plant Germplasm Resources, College of Life Sciences, Shanghai Normal University, Shanghai 200234, China.
  • 3 School of Life Sciences, East China Normal University, Shanghai 200241, China; School of Life Sciences, Anhui Agricultural University, Hefei 230036, China.
  • 4 School of Life Sciences, East China Normal University, Shanghai 200241, China; Institute of Eco-Chongming, East China Normal University, Shanghai 202162, China. Electronic address: cli@bio.ecnu.edu.cn.
PMID: 39793583 DOI: 10.1016/j.devcel.2024.12.026
Abstract

During pollen-stigma interaction, pollen coat protein B-class Peptides (PCP-Bs) compete with stigmatic rapid alkalinization factor (RALF) for interaction with FERONIA/ANJEA receptor kinases (FER/ANJ), stimulating pollen hydration and germination. However, the molecular mechanism underlying PCP-Bs-induced, FER/ANJ-regulated compatible responses remains largely unknown. Through PCP-Bγ-induced phosphoproteomic analysis, we characterized a series of pollination-related signaling pathways regulated by FER/ANJ. Interestingly, on stigmatic papillary cells, pollen PCP-Bγ induced an elevation in cytosolic pH near the plasma membrane (PM), sustained by stigmatic RALF23/33 through regulation of the autoinhibited H+-ATPase 1/2 (AHA1/2) activity. We further found that RALFs/PCP-Bs and FER/ANJ regulated the pH alterations via phosphorylation of AHA1/2 C terminus. Furthermore, RALF23/33-FER/ANJ maintained the protonation of H197 in plasma membrane intrinsic proteins (PIPs), whereas PCP-B relieved the protonation through AHA activity. Altogether, this study reveals that pollen PCP-Bs trigger FER/ANJ-controlled compatible responses, particularly the opening of aquaporins via AHA-mediated pH changes, thereby facilitating pollen hydration in Arabidopsis.

Keywords

FERONIA; H(+)-ATPase; PCP-B; aquaporins; compatible responses.

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