Nigericin sodium salt

Name: Nigericin sodium salt
Brand: MedChemExpress
SKU: HY-100381
Rating: 5.0 (133 reviews)

Cat. No.: HY-100381 Purity: 99.37%: FAQs
Data Sheet SDS COA Handling Instructions Technical Support

Nigericin sodium salt is an antibiotic derived from Streptomyces hygroscopicus that act as a K⁺/H⁺ ionophore, promoting K⁺/H⁺ exchange across mitochondrial membranes. Nigericin sodium salt is a NLRP3 activator. Nigericin sodium salt shows promising anti-cancer activities through decreasing intracellular pH (pHi), and inactivation of Wnt/β-catenin signals. Nigericin sodium salt induces pyroptosis through caspase 1/GSDMD in TNBC.

For research use only. We do not sell to patients.

Nigericin sodium salt Chemical Structure

CAS No. : 28643-80-3

Size	Stock	Quantity
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Based on 133 publication(s) in Google Scholar

Other Forms of Nigericin sodium salt:

Nigericin Get quote
Nigericin (sodium salt) (Standard) Get quote

130 Publications Citing Use of MCE Nigericin sodium salt

: Nigericin sodium salt purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2023 Mar 5;113:154743. [Abstract]; Priming with LPS (1 μg/mL; 4 h) and activating with Nigericin (10 μM; 2 h) induces NLRP3 expression, caspase-1 cleavage, and GSDMD cleavage in BMDMs.

: Nigericin sodium salt purchased from MedChemExpress. Usage Cited in: Environ Toxicol. 2023 Mar 29. [Abstract]; Nigericin (1.38mM; 2 μg/2 μL; 10 min) signifcantly increases the expression of IL-1β, IL-18, and GSDMD N-terminal in rats

: Nigericin sodium salt purchased from MedChemExpress. Usage Cited in: J Cell Mol Med. 2020 Jul;24(14):8078-8090. [Abstract]; LPS + Nigericin is used to induce pyroptosis as a positive control group.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

NLRP3

In Vitro

Nigericin (0-4 μg/mL; 24 h) sodium salt induces pyroptosis through caspase 1/GSDMD pathway in TNBC cells^[6].
Nigericin (1 μg/mL, 8 × MIC; 2 μg/mL, 16 × MIC; 196 h) sodium salt is a bactericidal antibiotic against MDR gram-positive bacteria^[4].
Nigericin (0-0.25 μg/mL; 24 h) sodium salt inactivates Wnt/β-catenin pathway in H460 cells as an anti-cancer effect^[7].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[4]

Cell Line:	S. aureus, Staphylococcus epidermidis, Enterococcus faecalis, E. faecium, S. pneumoniae) , and Streptococcus agalactiae
Concentration:	0 μg/mL, 0.05 μg/mL, 0.125 μg/mL, 0.25 μg/mL
Incubation Time:	24 h
Result:	Exhibited potent activity against these clinical MDR strains, with MIC values ranging from 0.004-0.25 mg/mL.

Western Blot Analysis^[6]

Cell Line:	MDA-MB-231, and 4T1 cells
Concentration:	0 μg/mL, 2 μg/mL, 4 μg/mL
Incubation Time:	24 h
Result:	Showed increasing in the proteins level of caspase 1 and N-GSDMD.

In Vivo

Nigericin (1 mg/kg; i.p.; every 12 h for 3 D) sodium salt reduces the infection of S. aureus USA300 in mice^[4].
Nigericin (0.025 mg/kg; s.c.; every two days in 4 weeks) sodium salt plus anti-PD-1 shows synergistic anti-cancer effect^[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Mice Infected with S. aureus USA300^[4]
Dosage:	1 mg/kg
Administration:	Intraperitoneal injection (i.p.)
Result:	Showed reduction in the bacterial burden to 1,000-10,000-fold in the major organs.

Animal Model:	BALB/c Mice injected orthotopically with 4T1 cells^[6]
Dosage:	0.025 mg/kg
Administration:	Subcutaneous injection (s.c.)
Result:	Showed combination with anti-PD-1 antibody almost completely suppressed tumor growth.

Molecular Weight

746.94

Formula

C₄₀H₆₇NaO₁₁

CAS No.

28643-80-3

Appearance

Solid

Color

White to off-white

SMILES

C[C@H]1[C@](O[C@@H]2C[C@](CC[C@@H]3C)([H])O[C@@]3([H])[C@@H](C)C(O[Na])=O)([C@@H]([C@H](OC)C2)C)O[C@](C)([C@@]4([H])O[C@@](C)([C@@]5([H])O[C@@]([C@]([C@@H](C[C@@H]6C)C)([H])O[C@]6(O)CO)([H])C[C@H]5C)CC4)C1

Structure Classification

Initial Source

Microorganisms

Streptomyces hygroscopicus

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

Ethanol : 25 mg/mL (33.47 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.3388 mL	6.6940 mL	13.3880 mL
5 mM	0.2678 mL	1.3388 mL	2.6776 mL
10 mM	0.1339 mL	0.6694 mL	1.3388 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% EtOH 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (3.35 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% EtOH 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (3.35 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% EtOH 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (3.35 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.37%

Select Batch:

Data Sheet (282 KB) SDS (480 KB)

COA (251 KB) HNMR (284 KB) RP-HPLC (247 KB)

Handling Instructions (2659 KB)

References

[1]. Zotova L, et al. Novel components of an active mitochondrial K(+)/H(+) exchange. J Biol Chem. 2010 May 7;285(19):14399-414. [Content Brief]

[2]. Mariathasan S, et al.Cryopyrin activates the inflammasome in response to toxins and ATP.Nature. 2006 Mar 9;440(7081):228-32. [Content Brief]

[3]. Bissinger R, et al. Triggering of Suicidal Erythrocyte Death by the Antibiotic Ionophore Nigericin. Basic Clin Pharmacol Toxicol. 2016 May;118(5):381-9. [Content Brief]

[4]. Xiaoli Zhu, et al. Nigericin is effective against multidrug resistant gram-positive bacteria, persisters, and biofilms. Front Cell Infect Microbiol. 2022 Dec 20:12:1055929. [Content Brief]

[5]. Guanzhuang Gao, et al. Evidence of nigericin as a potential therapeutic candidate for cancers: A review. Biomed Pharmacother. 2021 May:137:111262. [Content Brief]

[6]. Lisha Wu, et al. Nigericin Boosts Anti-Tumor Immune Response via Inducing Pyroptosis in Triple-Negative Breast Cancer. Cancers (Basel). 2023 Jun 16;15(12):3221. [Content Brief]

[7]. Juan Sebastian Yakisich, et al. Nigericin decreases the viability of multidrug-resistant cancer cells and lung tumorspheres and potentiates the effects of cardiac glycosides. Tumour Biol.2017 Mar;39(3):1010428317694310. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

Ethanol	1 mM	1.3388 mL	6.6940 mL	13.3880 mL	33.4699 mL
	5 mM	0.2678 mL	1.3388 mL	2.6776 mL	6.6940 mL
	10 mM	0.1339 mL	0.6694 mL	1.3388 mL	3.3470 mL
	15 mM	0.0893 mL	0.4463 mL	0.8925 mL	2.2313 mL
	20 mM	0.0669 mL	0.3347 mL	0.6694 mL	1.6735 mL
	25 mM	0.0536 mL	0.2678 mL	0.5355 mL	1.3388 mL
	30 mM	0.0446 mL	0.2231 mL	0.4463 mL	1.1157 mL