2. Academic Validation
  3. The role of 5-hydroxytryptamine on expulsion of Trichinella spiralis during the intestinal stage

The role of 5-hydroxytryptamine on expulsion of Trichinella spiralis during the intestinal stage

  • Vet Parasitol. 2025 Feb:334:110396. doi: 10.1016/j.vetpar.2025.110396.
Tongxuan Zhang 1,  Zhiyu Zhu 1,  Zhiyuan Zhang 1,  Xueting Wang 1,  Xuanrui Liu 1,  Wentao Yang 1,  Xin Cao 1,  Yanlong Jiang 1,  Jianzhong Wang 1,  Yan Zeng 1,  Chunwei Shi 1,  Haibin Huang 1,  Chunfeng Wang 1,  Nan Wang 2,  Guilian Yang 3
Affiliations

Affiliations

  • 1 College of Veterinary Medicine, Jilin Agricultural University, Changchun 130118, China; Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Provincial Key Laboratory of Animal Microecology and Healthy Breeding, Jilin Agricultural University, Changchun 130118, China; Engineering Research Center of Microecological Vaccines (Drugs) for Major Animal Diseases, Ministry of Education, Jilin Agricultural University, Changchun 130118, China.
  • 2 College of Veterinary Medicine, Jilin Agricultural University, Changchun 130118, China; Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Provincial Key Laboratory of Animal Microecology and Healthy Breeding, Jilin Agricultural University, Changchun 130118, China; Engineering Research Center of Microecological Vaccines (Drugs) for Major Animal Diseases, Ministry of Education, Jilin Agricultural University, Changchun 130118, China. Electronic address: wangnan2020@jlau.edu.cn.
  • 3 College of Veterinary Medicine, Jilin Agricultural University, Changchun 130118, China; Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Provincial Key Laboratory of Animal Microecology and Healthy Breeding, Jilin Agricultural University, Changchun 130118, China; Engineering Research Center of Microecological Vaccines (Drugs) for Major Animal Diseases, Ministry of Education, Jilin Agricultural University, Changchun 130118, China. Electronic address: yangguilian@jlau.edu.cn.
PMID: 39837238 DOI: 10.1016/j.vetpar.2025.110396
Abstract

During the intestinal stage of Trichinella spiralis (T. spiralis) Infection, it can stimulate host's intestinal peristalsis to expulse worms. 5-hydroxytryptamine (5-HT) is a neurotransmitter which can regulate the contraction of intestinal smooth muscle. IL-33 specifically binds to ST2 receptor to promote the secretion of 5-HT by intestinal enterochromaffin cells. However, it remains unclear whether the host is able to modulate the secretion of 5-HT to expulse worms through the IL-33-ST2 signaling pathway during the intestinal stage of T. spiralis Infection. Therefore, ST2 inhibitor iST2 was used in a T. spiralis infected mouse model and MODE-K cells to analyze the role of IL-33-ST2 signaling pathway in the secretion of 5-HT during the intestinal phase of T. spiralis Infection. The results indicated that the expression of ST2, IL-33, and TPH1(Tryptophan Hydroxylase 1, the rate-limiting Enzyme in the biosynthesis of 5-HT) in the small intestine were increased during the intestinal phase of T. spiralis Infection. Meanwhile the levels of secretory 5-HT and IL-33 in the small intestine were significantly increased. After iST2 treatment, the level of 5-HT was significantly decreased, resulting in diminished worms expulsion capability. The decrease of 5-HT was observed in MODE-K cells treated with excretory-secretory products of T. spiralis post iST2 treatment. The above results demonstrated that IL-33-ST2 signaling pathway might play a crucial role in promoting the secretion of 5-HT which enhancing the ability of the intestine to expulse worms during the intestinal stage of T. spiralis.

Keywords

5-HT; IL-33; ST2; Trichinella spiralis; Worm expulsion.

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