2. Academic Validation
  3. Protocol for oral fecal gavage to reshape the gut microbiota in mice

Protocol for oral fecal gavage to reshape the gut microbiota in mice

  • STAR Protoc. 2025 Jan 23;6(1):103585. doi: 10.1016/j.xpro.2024.103585.
Chun-Ju Yang 1,  Yi-Shian Peng 1,  Pin-Cheng Sung 1,  Sen-Yung Hsieh 2
Affiliations

Affiliations

  • 1 Department of Gastroenterology and Hepatology, Chang Gung Memorial Hospital, Linkou, Taoyuan, Taiwan.
  • 2 Department of Gastroenterology and Hepatology, Chang Gung Memorial Hospital, Linkou, Taoyuan, Taiwan; Grandulate Institute of Biomedical Science, College of Medicine, Chang Gung University, Taoyuan, Taiwan. Electronic address: siming@cgmh.org.tw.
PMID: 39854205 DOI: 10.1016/j.xpro.2024.103585
Abstract

Fecal microbiota transplantation (FMT) is clinically applied, while oral FMT (oral fecal gavage [OFG]) is preferred for experimental mice. Here, we present a protocol for OFG in antibiotic-pretreated mice, demonstrating the progressive, time-dependent evolution of the gut microbiota in the recipients. We describe steps for fecal sample collection and preparation procedures, oral gavage, and monitoring gut microbiota changes. This protocol serves as a general guide for reshaping the gut microbiota in recipient mice for various experimental applications. For complete details on the use and execution of this protocol, please refer to Yang et al.1.

Keywords

Biotechnology and bioengineering; Health sciences; Metabolism; Microbiology; Model organisms.

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