2. Academic Validation
  3. Adenylate cyclase 10 promotes brown adipose tissue thermogenesis

Adenylate cyclase 10 promotes brown adipose tissue thermogenesis

  • iScience. 2025 Jan 19;28(2):111833. doi: 10.1016/j.isci.2025.111833.
Anupam Das 1,  Christine Mund 2,  Eman Hagag 2,  Ruben Garcia-Martin 2,  Eleftheria Karadima 2,  Anke Witt 1,  Mirko Peitzsch 2,  Andreas Deussen 1,  Triantafyllos Chavakis 2 3 4,  Thomas Noll 1,  Vasileia Ismini Alexaki 2
Affiliations

Affiliations

  • 1 Department of Physiology, Faculty of Medicine, Technische Universität Dresden, Dresden, Germany.
  • 2 Institute for Clinical Chemistry and Laboratory Medicine, Faculty of Medicine and University Hospital Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany.
  • 3 Paul Langerhans Institute Dresden of the Helmholtz Center Munich, University Hospital and Faculty of Medicine, Technische Universität Dresden, Dresden, Germany.
  • 4 German Center for Diabetes Research (DZD), Neuherberg, Germany.
PMID: 39949963 DOI: 10.1016/j.isci.2025.111833
Abstract

Brown adipose tissue (BAT) thermogenesis dissipates energy through heat production and thereby it opposes Metabolic Disease. It is mediated by mitochondrial membrane uncoupling, yet the mechanisms sustaining the mitochondrial membrane potential (ΔΨm) in brown adipocytes are poorly understood. Here we show that isocitrate dehydrogenase (IDH) activity and the expression of the soluble Adenylate Cyclase 10 (ADCY10), a CO2/bicarbonate sensor residing in mitochondria, are upregulated in BAT of cold-exposed mice. IDH inhibition or ADCY10 deficiency reduces cold resistance of mice. Mechanistically, IDH increases the ΔΨm in brown adipocytes via ADCY10. ADCY10 sustains complex I activity and the ΔΨm via exchange protein activated by cAMP1 (EPAC1). However, neither IDH nor ADCY10 inhibition affect uncoupling protein 1 (UCP1) expression. Hence, we suggest that ADCY10, acting as a CO2/bicarbonate sensor, mediates the effect of IDH on complex I activity through cAMP-EPAC1 signaling, thereby maintaining the ΔΨm and enabling thermogenesis in brown adipocytes.

Keywords

Cell biology; Molecular biology; Physiology.

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