2. GPCR/G Protein
  3. Adenylate Cyclase
  4. KH7

KH7 est un inhibiteur spécifique de la adénylyl cyclase soluble (sAC), avec des IC50 de 3-10 μM vers les deux sACt humains purifiés recombinants protéine et sACt exprimés de façon hétérologue dans des tests cellulaires. KH7 est également un inhibiteur de cAMP.

KH7 is a soluble adenylyl cyclase (sAC)-specific inhibitor, with IC50s of 3-10 μM toward both recombinant purified human sACt protein and heterologously expressed sACt in cellular assays. KH7 is also a cAMP inhibitor.

For research use only. We do not sell to patients.

KH7 Chemical Structure

KH7 Chemical Structure

CAS No. : 330676-02-3

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10 mM * 1 mL in DMSO
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Customer Review

Based on 5 publication(s) in Google Scholar

KH7 Related Antibodies

Top Publications Citing Use of Products

    KH7 purchased from MedChemExpress. Usage Cited in: J Cell Physiol. 2020 Dec;235(12):9510-9523.  [Abstract]

    The P‐Akt protein expression is measured by Western blot analysis. The improved effects of PCTR1 on P‐AKT are partially blocked by KH7.

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    KH7 is a soluble adenylyl cyclase (sAC)-specific inhibitor, with IC50s of 3-10 μM toward both recombinant purified human sACt protein and heterologously expressed sACt in cellular assays[1]. KH7 is also a cAMP inhibitor[2].

    IC50 & Target

    IC50: 3-10 μM (recombinant sACt)[1].
    In Vitro

    KH7 (10 μM) blocks this capacitation-induced cAMP increase. At higher concentrations (50 μM), 5- to 10-fold above its IC50 but still selective for sAC relative to tmACs, KH7 resulted in a significant decrease in the basal cAMP accumulation in sperm regardless of the incubation medium[1].
    KH7 prevents the generation of CaSF[2].
    In the presence of KH7, the myocytes exerts a negative inotropic effect (NIE) of approximately 20%, suggesting that sAC is involved in the normal generation of basal cardiac contractility[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    KH7 Related Antibodies
    Molecular Weight

    419.30

    Formula

    C17H15BrN4O2S
    CAS No.
    Appearance

    Solid

    Color

    White to light yellow

    SMILES

    CC(SC1=NC2=CC=CC=C2N1)C(N/N=C/C3=CC(Br)=CC=C3O)=O
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    -20°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    DMSO : 250 mg/mL (596.23 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.3849 mL 11.9246 mL 23.8493 mL
    5 mM 0.4770 mL 2.3849 mL 4.7699 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: 2.5 mg/mL (5.96 mM); Suspended solution; Need ultrasonic

      This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (4.96 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: ≥99.0%

    COA (246 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.3849 mL 11.9246 mL 23.8493 mL 59.6232 mL
    5 mM 0.4770 mL 2.3849 mL 4.7699 mL 11.9246 mL
    10 mM 0.2385 mL 1.1925 mL 2.3849 mL 5.9623 mL
    15 mM 0.1590 mL 0.7950 mL 1.5900 mL 3.9749 mL
    20 mM 0.1192 mL 0.5962 mL 1.1925 mL 2.9812 mL
    25 mM 0.0954 mL 0.4770 mL 0.9540 mL 2.3849 mL
    30 mM 0.0795 mL 0.3975 mL 0.7950 mL 1.9874 mL
    40 mM 0.0596 mL 0.2981 mL 0.5962 mL 1.4906 mL
    50 mM 0.0477 mL 0.2385 mL 0.4770 mL 1.1925 mL
    60 mM 0.0397 mL 0.1987 mL 0.3975 mL 0.9937 mL
    80 mM 0.0298 mL 0.1491 mL 0.2981 mL 0.7453 mL
    100 mM 0.0238 mL 0.1192 mL 0.2385 mL 0.5962 mL
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    KH7 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    KH7330676-02-3KH 7KH-7Adenylate CyclaseAdenylyl cyclaseInhibitorinhibitorinhibit

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