2. GPCR/G Protein
  3. Adenylate Cyclase
  4. LRE1

LRE1 is a specific and allosteric inhibitor of soluble adenylyl cyclase.

For research use only. We do not sell to patients.

LRE1 Chemical Structure

LRE1 Chemical Structure

CAS No. : 1252362-53-0

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 77 In-stock
Solution
10 mM * 1 mL in DMSO USD 77 In-stock
Solid
5 mg USD 70 In-stock
10 mg USD 120 In-stock
50 mg USD 430 In-stock
100 mg USD 745 In-stock
200 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

LRE1 Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE LRE1

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

LRE1 is a specific and allosteric inhibitor of soluble adenylyl cyclase.

IC50 & Target

Adenylyl cyclase[1]
In Vitro

LRE1 binds to the bicarbonate activator binding site and inhibits soluble adenylyl cyclase (sAC) via a unique allosteric mechanism[1].

LRE1 prevents sAC-dependent processes in cellular and physiological systems and facilitates exploration of the therapeutic potential of sAC inhibition[1].

LRE1 (0.5-100 μM, 60 minutes ) inhibits sperm and mitochondrial functions of sAC[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

LRE1 Related Antibodies

Western Blot Analysis[1]

Cell Line: Mouse cauda sperm
Concentration: 0.5-100 µM
Incubation Time: 60 minutes
Result: LRE1 inhibits sAC dependent sperm capacitation[1].
Molecular Weight

280.78

Formula

C12H13ClN4S
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

NC1=NC(N(CC2=CC=CS2)C3CC3)=CC(Cl)=N1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (445.19 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.5615 mL 17.8075 mL 35.6151 mL
5 mM 0.7123 mL 3.5615 mL 7.1230 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.08 mg/mL (7.41 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.08 mg/mL (7.41 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.76%

COA (250 KB) LCMS (265 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.5615 mL 17.8075 mL 35.6151 mL 89.0377 mL
5 mM 0.7123 mL 3.5615 mL 7.1230 mL 17.8075 mL
10 mM 0.3562 mL 1.7808 mL 3.5615 mL 8.9038 mL
15 mM 0.2374 mL 1.1872 mL 2.3743 mL 5.9358 mL
20 mM 0.1781 mL 0.8904 mL 1.7808 mL 4.4519 mL
25 mM 0.1425 mL 0.7123 mL 1.4246 mL 3.5615 mL
30 mM 0.1187 mL 0.5936 mL 1.1872 mL 2.9679 mL
40 mM 0.0890 mL 0.4452 mL 0.8904 mL 2.2259 mL
50 mM 0.0712 mL 0.3562 mL 0.7123 mL 1.7808 mL
60 mM 0.0594 mL 0.2968 mL 0.5936 mL 1.4840 mL
80 mM 0.0445 mL 0.2226 mL 0.4452 mL 1.1130 mL
100 mM 0.0356 mL 0.1781 mL 0.3562 mL 0.8904 mL
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LRE1 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

LRE11252362-53-0LRE 1LRE-1Adenylate CyclaseAdenylyl cyclaseInhibitorinhibitorinhibit

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