2. Academic Validation
  3. Glycolysis regulates palatal mesenchyme proliferation through Pten-Glut1 axis via Pten classical and non-classical pathways

Glycolysis regulates palatal mesenchyme proliferation through Pten-Glut1 axis via Pten classical and non-classical pathways

  • Cell Biol Toxicol. 2025 Feb 27;41(1):53. doi: 10.1007/s10565-025-10000-2.
Yijia Wang 1 2,  Xia Peng 1,  Xiaotong Wang 1,  Jing Chen 1,  Xiaoyu Zheng 1,  Xige Zhao 1,  Cui Guo 3,  Juan Du 4
Affiliations

Affiliations

  • 1 Laboratory of Orofacial Development, Laboratory of Molecular Signaling and Stem Cells Therapy, Molecular Laboratory for Gene Therapy and Tooth Regeneration, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Capital Medical University School of Stomatology, No.9 Fanjiacun Road, Beijing, 100070, China.
  • 2 Laboratory of Tissue Regeneration and Immunology and Department of Periodontics, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Capital Medical University School of Stomatology, No.9 Fanjiacun Road, Beijing, 100070, China.
  • 3 Department of Geriatric Dentistry, Capital Medical University School of Stomatology, Fanjiacun Road No.9, Beijing, 100070, China.
  • 4 Laboratory of Orofacial Development, Laboratory of Molecular Signaling and Stem Cells Therapy, Molecular Laboratory for Gene Therapy and Tooth Regeneration, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Capital Medical University School of Stomatology, No.9 Fanjiacun Road, Beijing, 100070, China. juandug@ccmu.edu.cn.
PMID: 40014184 DOI: 10.1007/s10565-025-10000-2
Abstract

Abnormal embryonic development leads to the formation of cleft palate (CP) which is difficult to be detected by genetic screening and needs sequent treatment from infants to adults. There are no interceptive treatment about CP until now. Germline deletion of Phosphatase and tensin homolog (PTEN) was related to embryonic malformation and regulated tumor cell proliferation through glycolysis. However, the role of PTEN in CP and the relationship between CP, PTEN, and glycolysis are unknown. In our research, we constructed PTEN knockdown models in vitro and in vivo. Our results provided preliminary evidence that blocking PTEN by its inhibitor such as VO-OHpic might be an effective interceptive treatment in early period of palate development when pregnant mother expose in harmful environment during the early period of palate development to reducing CP occurring which was related with the crosstalk between PTEN, and glycolysis in the process.

Keywords

Cleft palate; Glycolysis; Interceptive treatment; Proliferation; Pten.

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