2. Academic Validation
  3. The novel GSDMD inhibitor GI-Y2 exerts antipyroptotic effects to reduce atherosclerosis

The novel GSDMD inhibitor GI-Y2 exerts antipyroptotic effects to reduce atherosclerosis

  • Clin Transl Med. 2025 Mar;15(3):e70263. doi: 10.1002/ctm2.70263.
Xiaoxi Fan 1 2,  Zhenfeng Cheng 3,  Ruiyin Shao 1,  Keke Ye 1,  Xudong Chen 1,  Xueli Cai 1,  Shanshan Dai 4,  Zhixuan Tang 5,  Si Shi 5,  Wenyuan Zheng 6,  Weijian Huang 1,  Jibo Han 2,  Bozhi Ye 1
Affiliations

Affiliations

  • 1 Department of Cardiology and The Key Laboratory of Cardiovascular Disease of Wenzhou, the First Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, China.
  • 2 Department of Cardiology, The Second Affiliated Hospital of Jiaxing University, Jiaxing, Zhejiang, China.
  • 3 Huzhou Central Hospital, Affiliated Central Hospital of Huzhou University, Huzhou, China.
  • 4 The Key Laboratory of Emergency and Disaster Medicine of Wenzhou, Department of Emergency, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China.
  • 5 First School of Medicine, Wenzhou Medical University, Wenzhou, Zhejiang, China.
  • 6 Key Laboratory of Precision Medicine For Atherosclerosis Disease of Zhejiang Province, Department of Cardiology, Affiliated First Hospital of Ningbo University, Ningbo, Zhejiang, China.
PMID: 40045452 DOI: 10.1002/ctm2.70263
Abstract

Introduction: Gasdermin D (GSDMD) and the Pyroptosis it mediates are importantly involved in cardiovascular diseases (CVDs). Identifying and developing new inhibitors of GSDMD could be a promising strategy for treating pyroptosis-mediated diseases, such as atherosclerosis.

Objectives: We aimed to develop new inhibitor of GSDMD in atherosclerosis, as well as clarify the mechanisms underlying this inhibiting effect.

Methods: Surface plasmon resonance and pull-down assay were used to identify the amino acid sites of GSDMD inhibited by GI-Y2. A mouse model of atherosclerosis was established by feeding a high-fat diet for 12 weeks. After treating mice with GI-Y2 (10 or 20 mg/kg, i.g.), the lipid plaque area on the arterial intimal surface, lipid deposition, collagen deposition and Pyroptosis levels in aortic root sections were evaluated. Additionally, further treatment of atherosclerotic mice with macrophage membrane-encapsulated GI-Y2 was conducted to enhance the targeting ability of GI-Y2 to atherosclerotic plaques.

Results: In this study, we confirmed GI-Y2 as a novel inhibitor of GSDMD via structure-based virtual screening and pharmacological validation. Mechanistically, GI-Y2 directly interacts with the Arg10 residue of GSDMD and reduces the membrane binding of GSDMD-N. Functionally, we revealed that GI-Y2 inhibits the formation of atherosclerotic plaques by targeting GSDMD. Similarly, GI-Y2 reduces Pyroptosis and macrophage infiltration in atherosclerosis. Furthermore, we constructed macrophage membrane-coated GI-Y2 nanoparticles to enhance the targeting of GI-Y2 to macrophages in atheromatous plaques and demonstrated its vascular protective effect in vivo.

Conclusion: This work demonstrated that GI-Y2 can potentially alleviate CVDs by targeting GSDMD and provided a new compound for the study of GSDMD-mediated Pyroptosis.

Key points: We preliminarily confirmed GI-Y2 as a novel inhibitor of GSDMD via structure-based virtual screening and pharmacological validation. GI-Y2 directly interacts with GSDMD and reduces the membrane binding of GSDMD-N via the Arg10 residue. GI-Y2 inhibits the formation of atherosclerotic plaques by targeting GSDMD and GI-Y2 reduces Pyroptosis and macrophage infiltration in atherosclerosis. We constructed macrophage membrane-coated GI-Y2 nanoparticles to enhance the targeting of GI-Y2 to macrophages in atheromatous plaques and demonstrated its vascular protective effect in vivo.

Keywords

atherosclerosis; gasdermin D; macrophage; pyroptosis.

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