2. Academic Validation
  3. LncRNA TCONS_00067339 as a key regulatory factor inducing decreased cell viability and ferroptosis in neonatal hypoxic-ischemic brain damage

LncRNA TCONS_00067339 as a key regulatory factor inducing decreased cell viability and ferroptosis in neonatal hypoxic-ischemic brain damage

  • Brain Res. 2025 May 1:1854:149562. doi: 10.1016/j.brainres.2025.149562.
Yishi Li 1,  Junfang Sun 1,  Chunchi Lai 1,  Ting Li 2,  Lulu Zhang 2,  Feng Zhang 1,  Shiyi Ma 1,  Mengya Sun 3,  Hong Jiang 4
Affiliations

Affiliations

  • 1 Department of Pediatric, The Affiliated Hospital of Qingdao University, Qingdao, Shandong Province 266003, China; Animal Experiment Center, Central Laboratory, The Affiliated Hospital of Qingdao University, Qingdao, Shandong Province 266003, China.
  • 2 Department of Pediatric, The Affiliated Hospital of Qingdao University, Qingdao, Shandong Province 266003, China.
  • 3 Department of Pediatric, The Affiliated Hospital of Qingdao University, Qingdao, Shandong Province 266003, China. Electronic address: sunmengya616@163.com.
  • 4 Department of Pediatric, The Affiliated Hospital of Qingdao University, Qingdao, Shandong Province 266003, China; Animal Experiment Center, Central Laboratory, The Affiliated Hospital of Qingdao University, Qingdao, Shandong Province 266003, China. Electronic address: jianghongbs@163.com.
PMID: 40058623 DOI: 10.1016/j.brainres.2025.149562
Abstract

Newborn hypoxic-ischemic brain damage (HIBD) is a major cause of mortality and neurological disabilities. Ferroptosis, characterized by lipid peroxidation, is implicated in HIBD pathogenesis. The role of lncRNA TCONS_00067339 in Ferroptosis regulation in HIBD is understudied. This study investigates its mechanisms using a HIBD rat model and PC12 high differentiation cells oxygen-glucose deprivation (OGD) model. We identified upregulated lncRNA TCONS_00067339 in HIBD, associated with cells viability and ferroptosis-related mitochondrial changes. RNA Sequencing revealed differential lncRNA expression in hippocampal, and enrichment analyses suggested involvement in Ferroptosis pathways. Knockdown of lncRNA TCONS_00067339 increased OGD-treated PC12 cells viability and reduced cell death. These findings indicate that lncRNA TCONS_00067339 is a key regulator in Ferroptosis and cell survival in HIBD, offering a potential target for therapeutic intervention.

Keywords

Ferroptosis; Hypoxic-ischemic brain damage (HIBD); Oxygen-glucose deprivation (OGD); lncRNAs.

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