2. Academic Validation
  3. Evaluation of the Apoptotic, Prooxidative and Therapeutic Effects of Odoroside A on Lung Cancer: An In Vitro Study Extended with In Silico Analyses of Human Lung Cancer Datasets

Evaluation of the Apoptotic, Prooxidative and Therapeutic Effects of Odoroside A on Lung Cancer: An In Vitro Study Extended with In Silico Analyses of Human Lung Cancer Datasets

  • Life (Basel). 2025 Mar 12;15(3):445. doi: 10.3390/life15030445.
Fatma Seçer Çelik 1,  Göksemin Fatma Şengül 2,  Safaa Altveş 3,  Canan Eroğlu Güneş 4
Affiliations

Affiliations

  • 1 Department of Medical Biology and Genetics, Faculty of Medicine, Ankara Medipol University, 06050 Ankara, Turkey.
  • 2 Department of Medical Biochemistry, Faculty of Medicine, Ankara Medipol University, 06050 Ankara, Turkey.
  • 3 Science and Technology Research and Application Center (BITAM), Necmettin Erbakan University, 42005 Konya, Turkey.
  • 4 Department of Medical Biology, Faculty of Medicine, Necmettin Erbakan University, 42005 Konya, Turkey.
PMID: 40141789 DOI: 10.3390/life15030445
Abstract

Objective: The apoptotic effects of odoroside A on lung Cancer cells were studied in our project. We also supported and extended our experimentally-proven results via bioinformatics analysis on human lung Cancer tissues.

Materials and methods: In vitro studies were conducted using the A549 cell line. Cell proliferation was evaluated through a CCK-8 assay. For gene expression analysis, the qRT-PCR method was used, while CASP3 protein levels were detected using Western blotting and ELISA. Moreover, the oxidant status of cells was determined by measuring TAS and TOS levels. To construct a protein-protein interaction network, STRING analysis was performed. For the determination of differential expression of apoptosis-related genes, the GEPIA tool was utilized. Kaplan-Meier plots with overall survival, disease-specific survival and progression free intervals were obtained from UCSC Xena to evaluate the prognostic value of caspases.

Results: The gene expression levels of CASP3, CASP7, CASP8, CASP9, FAS, and FADD were elevated between 4-16 fold in Odo A-treated lung Cancer cells compared to controls. CASP3 protein expression was significantly higher in Odo A-treated cancerous cells than the control group. Low TAS (0.5700 ± 0.0067 in Odo A vs. 0.6437 ± 0.0151 in control) and high TOS (0.82800 ± 0.0208 in Odo A vs. 0.6263 ± 0.0258 in control) levels as well as high OSI values (1.4531 ± 0.0414 in Odo A vs. 0.9748 ± 0.0539 in control) were obtained. Correlogram and protein-protein network analyses suggested strong correlations and interactions among target genes. Lastly, Kaplan-Meier analysis showed no prognostic value of caspases, but potential therapeutic targets in lung Cancer.

Conclusions: Anti-cancer, prooxidative and therapeutic effects of Odo A on lung Cancer cells were shown in our study. These data were supported and extended via computational analyses that we performed. In conclusion, Odo A could be used in clinics to treat patients with lung Cancer.

Keywords

CASP3; apoptosis; lung cancer; odoroside A; redox hemostasis.

Figures
Products