2. Academic Validation
  3. The interaction of anthraquinone dyes with the plasmid-mediated OXA-2 beta-lactamase

The interaction of anthraquinone dyes with the plasmid-mediated OXA-2 beta-lactamase

  • Biochem J. 1982 Aug 1;205(2):413-7. doi: 10.1042/bj2050413.
C Monaghan,  S Holland,  J W Dale
PMID: 6982708 DOI: 10.1042/bj2050413
Abstract

Although beta-lactamases do not require any nucleotide co-substrates, the OXA-2 type is inhibited competitively by Cibacron Blue 3GA, and by other anthraquinone dyes, including some simpler compounds with no side chain. The Enzyme causes a red shift in the spectrum of Cibacron Blue. The Beta-lactamase can be adsorbed in Blue Sepharose and specifically eluted by benzylpenicillin. These results indicate that the binding of anthraquinone dyes is a specific effect similar to that seen with many nucleotide-binding Enzymes.

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