2. Others Anti-infection
  3. Fluorescent Dye Beta-lactamase
  4. Cibacron Blue 3G-A

Cibacron Blue 3G-A is an anthraquinone dye, inhibits the R46 β-lactamase with a Ki value of 1.2 uM.

For research use only. We do not sell to patients.

Cibacron Blue 3G-A Chemical Structure

Cibacron Blue 3G-A Chemical Structure

CAS No. : 84166-13-2

Size Price Stock Quantity
10 mg USD 50 In-stock
25 mg USD 70 In-stock
50 mg USD 90 In-stock
100 mg USD 150 In-stock
200 mg   Get quote  
500 mg   Get quote  

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Cibacron Blue 3G-A Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Cibacron Blue 3G-A

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Metallo-β-lactamase

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Cibacron Blue 3G-A is an anthraquinone dye, inhibits the R46 β-lactamase with a Ki value of 1.2 uM[1].

IC50 & Target

IC50: anthraquinone dye[1]
In Vitro

Cibacron Blue 3G-A is a structural analogy between the dye and NADH, it interacts with (di)nucleotide-dependent enzymes and can be a standard tool for studying their active sites[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cibacron Blue 3G-A Related Antibodies
Molecular Weight

774.16

Formula

C29H20ClN7O11S3
CAS No.
Appearance

Solid

Color

Brown to black

SMILES

O=S(C(C(N)=C1C2=O)=CC(NC3=CC=C(NC4=NC(Cl)=NC(NC5=CC=CC=C5S(=O)(O)=O)=N4)C(S(=O)(O)=O)=C3)=C1C(C6=C2C=CC=C6)=O)(O)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 62.5 mg/mL (80.73 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

1M NaOH : 10 mg/mL (12.92 mM; ultrasonic and adjust pH to 12 with NaOH)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.2917 mL 6.4586 mL 12.9172 mL
5 mM 0.2583 mL 1.2917 mL 2.5834 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.08 mg/mL (2.69 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 79.18%

COA (260 KB) HNMR (321 KB) LCMS (111 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
1M NaOH / DMSO 1 mM 1.2917 mL 6.4586 mL 12.9172 mL 32.2931 mL
5 mM 0.2583 mL 1.2917 mL 2.5834 mL 6.4586 mL
10 mM 0.1292 mL 0.6459 mL 1.2917 mL 3.2293 mL
DMSO 15 mM 0.0861 mL 0.4306 mL 0.8611 mL 2.1529 mL
20 mM 0.0646 mL 0.3229 mL 0.6459 mL 1.6147 mL
25 mM 0.0517 mL 0.2583 mL 0.5167 mL 1.2917 mL
30 mM 0.0431 mL 0.2153 mL 0.4306 mL 1.0764 mL
40 mM 0.0323 mL 0.1615 mL 0.3229 mL 0.8073 mL
50 mM 0.0258 mL 0.1292 mL 0.2583 mL 0.6459 mL
60 mM 0.0215 mL 0.1076 mL 0.2153 mL 0.5382 mL
80 mM 0.0161 mL 0.0807 mL 0.1615 mL 0.4037 mL
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Cibacron Blue 3G-A Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Cibacron Blue 3G-A84166-13-2Fluorescent DyeBeta-lactamaseβ-lactamaseAnthraquinoneDyelabelInhibitorinhibitorinhibit

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