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  3. Molecular cloning, sequencing and expression of a cDNA encoding a human liver NAD-dependent alpha-glycerol-3-phosphate dehydrogenase

Molecular cloning, sequencing and expression of a cDNA encoding a human liver NAD-dependent alpha-glycerol-3-phosphate dehydrogenase

  • Biochim Biophys Acta. 1995 May 17;1262(1):91-4. doi: 10.1016/0167-4781(95)00069-s.
J Menaya 1,  C González-Manchón,  R Parrilla,  M S Ayuso
Affiliations

Affiliation

  • 1 Department of Physiopathology and Human Molecular Genetics, Centro de Investigaciones Biológicas, CSIC, Madrid, Spain.
PMID: 7772607 DOI: 10.1016/0167-4781(95)00069-s
Abstract

This work reports the primary nucleotide structure and in vitro translation of a cDNA, expressed by a gene mapping on chromosome 12, that encodes a human hepatic alpha-glycerol-3-phosphate dehydrogenase (L-glycerol-3-phosphate:NAD oxidoreductase, E.C. 1.1.1.8). The 1413 bp cDNA comprises an ORF of 1050 bp that encodes a 349 amino acid protein of 37.5 kDa. Northern blot analysis of poly(A)+ mRNA from human liver showed three transcripts, while from human placenta only two transcripts were detected.

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