2. Academic Validation
  3. The effect of 6-dimethylaminopurine (6-DMAP) on DNA synthesis in activated mammalian oocytes

The effect of 6-dimethylaminopurine (6-DMAP) on DNA synthesis in activated mammalian oocytes

  • Zygote. 1996 Feb;4(1):7-9. doi: 10.1017/s0967199400002835.
S Ledda 1,  P Loi,  L Bogliolo,  R M Moor,  J Fulka Jr
Affiliations

Affiliation

  • 1 University of Sassari, Department of Physiology, Italy.
PMID: 8735365 DOI: 10.1017/s0967199400002835
Abstract

Mouse, sheep and bovine metaphase II oocytes were briefly preincubated in medium with 6-dimethylaminopurine (6-DMAP) and thereafter activated with ethanol before further culture in 6-DMAP supplemented medium. The presence of 6-DMAP enhances the efficiency of activation and the speed of pronuclear formation but has no effect on DNA synthesis. These results are discussed in the context of recently published data showing that the preincubation of condensed chromosomes with 6-DMAP blocks DNA synthesis when nuclei become reformed.

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