6-(Dimethylamino)purine (Synonyms: 6-Dimethylaminopurine; 6-DMAP)

Name: 6-(Dimethylamino)purine
Brand: MedChemExpress
SKU: HY-W010128
Rating: 4.5 (1 reviews)

Cat. No.: HY-W010128 Purity: 99.79%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

6-(Dimethylamino) purine (6-Dimethylaminopurine) is a serine threonine protein kinase inhibitor. 6-(Dimethylamino) purine can inhibit prolactin induced expression of lactoprotein genes in rabbit mammary gland cells. 6-(Dimethylamino) purine can affect the maturation of mammalian oocytes. 6-(Dimethylamino) purine can lead to downregulation of genes related to cell proliferation and cell cycle progression, such as proliferating cell nuclear antigen, insulin-like gene 1, and serine protease inhibitor 2 genes, and induce apoptosis in lymphoma cells (apoptosis).

For research use only. We do not sell to patients.

6-(Dimethylamino)purine Chemical Structure

CAS No. : 938-55-6

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 28	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 28	In-stock	Estimated Time of Arrival: December 31
Solid
100 mg	USD 25	In-stock	Estimated Time of Arrival: December 31
250 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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Purity & Documentation
References
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Description

IC₅₀ & Target

protein kinase^[1].

In Vitro

6-(Dimethylamino) purine (2.5 mM. 3 h) accelerates the transition to interphase in activated mouse oocytes by inhibiting protein kinases^[1].
6-(Dimethylamino) purine blocks DNA synthesis in activated mammalian oocytes^[2].
6-(Dimethylamino) purine inhibits prolactin induced expression of milk protein genes in rabbit mammary gland cells^[3].
6-(Dimethylamino) purine (0.5 mM, 6 h) affects sperm motility and vitality in mammals and insects by regulating protein phosphorylation non phosphorylation changes^[4].
6-(Dimethylamino) purine (5 mM, 16 h) induces apoptosis in lymphoma cells by inhibiting protein kinases, leading to a significant downregulation of all genes related to cell proliferation and cell cycle progression, such as proliferating cell nuclear antigen, insulin-derived gene 1, and serine protease inhibitor 2 genes^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1].

Cell Line:	Oocytes
Concentration:	2.5 mM
Incubation Time:	3 h
Result:	Induced protein dephosphorylation.

Western Blot Analysis^[4].

Cell Line:	The fly and mouse sperm
Concentration:	0.5 mM
Incubation Time:	6 h
Result:	Reduced the production of dynein and β - actin in fly sperm, while increasing the levels of both proteins in mice.

Apoptosis Analysis^[5].

Cell Line:	Human lymphoma U937 cell
Concentration:	5 mM
Incubation Time:	16 h
Result:	Induced apparent DNA fragmentation, phosphatidylserine externalization and lowering of the mitochondrial membrane potential and cell apoptosis.

Molecular Weight

163.18

Formula

C₇H₉N₅

CAS No.

938-55-6

Appearance

Solid

Color

White to off-white

SMILES

CN(C)C1=C2NC=NC2=NC=N1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 41.67 mg/mL (255.36 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	6.1282 mL	30.6410 mL	61.2820 mL
5 mM	1.2256 mL	6.1282 mL	12.2564 mL
10 mM	0.6128 mL	3.0641 mL	6.1282 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (12.75 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (12.75 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (12.75 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.79%

Select Batch:

Data Sheet (280 KB) SDS (393 KB)

COA (253 KB) HNMR (241 KB) LCMS (266 KB)

Handling Instructions (2659 KB)

References

[1]. Szöllösi MS, et al. Inhibition of protein kinases by 6-dimethylaminopurine accelerates the transition to interphase in activated mouse oocytes. J Cell Sci. 1993 Mar;104 ( Pt 3):861-72. [Content Brief]

[2]. Ledda S, et al. The effect of 6-dimethylaminopurine (6-DMAP) on DNA synthesis in activated mammalian oocytes. Zygote. 1996 Feb;4(1):7-9. [Content Brief]

[3]. Bayat-Sarmadi M, et al. 6-Dimethyl amino purine and 2-amino purine inhibit the induction of expression of milk protein genes by prolactin. Biosci Rep. 1992 Jun;12(3):189-97. [Content Brief]

[4]. Ogneva IV. Mouse and Fly Sperm Motility Changes Differently under Modelling Microgravity. Curr Issues Mol Biol. 2021 Jul 5;43(2):590-604. [Content Brief]

[5]. Nakashima M, et al. Apoptosis induced by an alkylated purine, 6-dimethylaminopurine, and changes in gene expression in human lymphoma U937 cells. Anticancer Res. 2008 Mar-Apr;28(2A):609-20. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	6.1282 mL	30.6410 mL	61.2820 mL	153.2051 mL
	5 mM	1.2256 mL	6.1282 mL	12.2564 mL	30.6410 mL
	10 mM	0.6128 mL	3.0641 mL	6.1282 mL	15.3205 mL
	15 mM	0.4085 mL	2.0427 mL	4.0855 mL	10.2137 mL
	20 mM	0.3064 mL	1.5321 mL	3.0641 mL	7.6603 mL
	25 mM	0.2451 mL	1.2256 mL	2.4513 mL	6.1282 mL
	30 mM	0.2043 mL	1.0214 mL	2.0427 mL	5.1068 mL
	40 mM	0.1532 mL	0.7660 mL	1.5321 mL	3.8301 mL
	50 mM	0.1226 mL	0.6128 mL	1.2256 mL	3.0641 mL
	60 mM	0.1021 mL	0.5107 mL	1.0214 mL	2.5534 mL
	80 mM	0.0766 mL	0.3830 mL	0.7660 mL	1.9151 mL
	100 mM	0.0613 mL	0.3064 mL	0.6128 mL	1.5321 mL

6-(Dimethylamino)purine Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

6-(Dimethylamino)purine938-55-66-Dimethylaminopurine 6-DMAPCDKDNA/RNA SynthesisApoptosisCyclin dependent kinaseprotein kinaseoocytelymphomasperm vitalityInhibitorinhibitorinhibit

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6-(Dimethylamino)purine (Synonyms: 6-Dimethylaminopurine; 6-DMAP)

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6-(Dimethylamino)purine Related Antibodies

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Complete Stock Solution Preparation Table

6-(Dimethylamino)purine Related Classifications

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