2. Academic Validation
  3. A general ribonuclease assay using methylene blue

A general ribonuclease assay using methylene blue

  • Anal Biochem. 1996 Aug 15;240(1):24-8. doi: 10.1006/abio.1996.0326.
T Greiner-Stoeffele 1,  M Grunow,  U Hahn
Affiliations

Affiliation

  • 1 Institut für Biochemie, Fakultät für Biowissenschaften, Pharmazie, und Psychologie, Universität Leipzig, Talstrasse 33, Leipzig, D-04103, Federal Republic of Germany.
PMID: 8811875 DOI: 10.1006/abio.1996.0326
Abstract

Ribonuclease (RNase) activity has been assayed by monitoring the shift in the absorbance maximum of methylene blue upon intercalation into high-molecular-weight RNA. After preincubation of yeast RNA with methylene blue, the initial rate of hydrolysis can be followed spectrophotometrically at a wavelength of 688 nm. This allows Enzyme kinetic studies of RNases with their probable native substrate, RNA, in place of the artificial ones, such as dinucleoside phosphates or cyclic monophosphates, currently used.

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