2. PROTAC Immunology/Inflammation
  3. PROTACs Macrophage migration inhibitory factor (MIF)
  4. MD13

MD13 is a macrophage migration inhibitory factor (MIF)-directed PROTAC with a Ki of 71 nM. MD13 can be used for cancer research.

For research use only. We do not sell to patients.

MD13 Chemical Structure

MD13 Chemical Structure

CAS No. : 2758431-97-7

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Based on 1 publication(s) in Google Scholar

MD13 Related Antibodies

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von Hippel-Lindau (VHL) Cereblon MDM2 cIAP1

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Description

MD13 is a macrophage migration inhibitory factor (MIF)-directed PROTAC with a Ki of 71 nM. MD13 can be used for cancer research[1].

IC50 & Target

Ki: 71 nM (MIF)[1]
In Vitro

MD13 degrades 91±5% and 71±7% MIF at 2 μM and 0.2 μM, respectively. MD13 induces MIF degradation through binding to E3 ligase cereblon[1].
MD13 (0-20 μM; 72 h) inhibits cell proliferation of A549 cancer cells[1].
MD13 (1-5 μM; 48 h) arrests cell cycle at G2/M phase in A549 cells[1].
MD13 (2 μM; 6-48 h) inhibits ERK signaling[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

MD13 Related Antibodies

Western Blot Analysis[1]

Cell Line: A549 cells
Concentration: 0-20 μM
Incubation Time: 12 h
Result: Caused depletion of MIF protein.

Western Blot Analysis[1]

Cell Line: A549 cells
Concentration: 2 μM
Incubation Time: 6, 24 or 48 h
Result: Inhibited ERK phosphorylation.

Cell Proliferation Assay[1]

Cell Line: A549 cells
Concentration: 0-20 μM
Incubation Time: 72 h
Result: Inhibited the growth of A549 cells in a dose-dependent manner. The inhibitory effect reached about 50% inhibition of cell proliferation at a concentration of 20 µM.

Cell Cycle Analysis[1]

Cell Line: A549 cells
Concentration: 1, 2, or 5 μM
Incubation Time: 48 h
Result: Dose-dependently induced cell cycle arrest at the G2/M phase.
Molecular Weight

653.68

Formula

C35H35N5O8
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C(NC1=CC=C(N2C(OC3=CC(O)=CC=C3C2)=O)C=C1)CCCCCCCNC4=CC=CC(C(N5C(CC6)C(NC6=O)=O)=O)=C4C5=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (152.98 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.5298 mL 7.6490 mL 15.2980 mL
5 mM 0.3060 mL 1.5298 mL 3.0596 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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In Vivo Dissolution Calculator
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Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.5298 mL 7.6490 mL 15.2980 mL 38.2450 mL
5 mM 0.3060 mL 1.5298 mL 3.0596 mL 7.6490 mL
10 mM 0.1530 mL 0.7649 mL 1.5298 mL 3.8245 mL
15 mM 0.1020 mL 0.5099 mL 1.0199 mL 2.5497 mL
20 mM 0.0765 mL 0.3825 mL 0.7649 mL 1.9123 mL
25 mM 0.0612 mL 0.3060 mL 0.6119 mL 1.5298 mL
30 mM 0.0510 mL 0.2550 mL 0.5099 mL 1.2748 mL
40 mM 0.0382 mL 0.1912 mL 0.3825 mL 0.9561 mL
50 mM 0.0306 mL 0.1530 mL 0.3060 mL 0.7649 mL
60 mM 0.0255 mL 0.1275 mL 0.2550 mL 0.6374 mL
80 mM 0.0191 mL 0.0956 mL 0.1912 mL 0.4781 mL
100 mM 0.0153 mL 0.0765 mL 0.1530 mL 0.3825 mL
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MD13 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

MD132758431-97-7MD 13MD-13PROTACsMacrophage migration inhibitory factor (MIF)A549MIFmacrophage migration inhibitory factoranticancerERK signalingInhibitorinhibitorinhibit

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